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Titolo:
Immunohistochemical distribution of enkephalin, substance P, and somatostatin in the brainstem of the leopard frog, Rana pipiens
Autore:
Stuesse, SL; Adli, DSH; Cruce, WLR;
Indirizzi:
NE Ohio Univ, Coll Med, Neurobiol & Pharmacol Dept, Rootstown, OH 44272 USA NE Ohio Univ Rootstown OH USA 44272 armacol Dept, Rootstown, OH 44272 USA Univ Malaya, Dept Zool, Kuala Lumpur 59100, Malaysia Univ Malaya Kuala Lumpur Malaysia 59100 ol, Kuala Lumpur 59100, Malaysia
Titolo Testata:
MICROSCOPY RESEARCH AND TECHNIQUE
fascicolo: 4, volume: 54, anno: 2001,
pagine: 229 - 245
SICI:
1059-910X(20010815)54:4<229:IDOESP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; NORTH-AMERICAN OPOSSUM; STEM RETICULOSPINAL NUCLEI; SPINAL-CORD; RETICULAR-FORMATION; TYROSINE-HYDROXYLASE; DESCENDING PATHWAYS; IMMUNOREACTIVE STRUCTURES; XENOPUS-LAEVIS; LEU-ENKEPHALIN;
Keywords:
enkephalin; substance P; somatostatin; reticulospinal; supraspinal; brainstem; amphibian;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
112
Recensione:
Indirizzi per estratti:
Indirizzo: Cruce, WLR NE Ohio Univ, Coll Med, Neurobiol & Pharmacol Dept, Rootstown, OH 44272 USA NE Ohio Univ Rootstown OH USA 44272 t, Rootstown, OH 44272 USA
Citazione:
S.L. Stuesse et al., "Immunohistochemical distribution of enkephalin, substance P, and somatostatin in the brainstem of the leopard frog, Rana pipiens", MICROSC RES, 54(4), 2001, pp. 229-245

Abstract

The brainstems of frogs contain many of the neurochemicals that are found in mammals. However, the clustering of nuclei near the ventricles makes it difficult to distinguish individual cell groups. We addressed this problem by combining immunohistochemistry with tract tracing and an analysis of cell morphology to localize neuropeptides within the brainstem of Rana pipiens. We injected a retrograde tracer, Fluoro-Gold, into the spinal cord, and, in the same frog, processed adjacent sections for immunohistochemical location of antibodies to the neuropeptides enkephalin (ENK), substance P (SP), and somatostatin (SOM). SOM+ cells were more widespread than cells containing immunoreactivity (ir) to the other substances. Most reticular nuclei in frog brainstem contained ir to at least one of these chemicals. Cells with SOM ir were found in nucleus (n.) reticularis pontis oralis, n. reticularismagnocellularis, n. reticularis paragigantocellularis, n. reticularis dorsalis, the optic tectum, n. interpeduncularis, and n. solitarius. ENK-containing cell bodies were found in n. reticularis pontis oralis, n. reticularisdorsalis, the nucleus of the solitary tract, and the tectum. The midbrain contained most of the SP+ cells. Six nonreticular nuclei (griseum centrale rhombencephali, n. isthmi, n. profundus mesencephali, n. interpeduncularis,torus semicircularis laminaris, and the tectum) contained ir to one or more of the substances but did not project to the spinal cord. The descending tract of V, and the rubrospinal, reticulospinal, and solitary tracts contained all three peptides as did the n. profundus mesencephali, n. isthmi, andspecific tectal layers. Because the distribution of neurochemicals within the frog brainstem is similar to that of amniotes, our results emphasize the large amount of conservation of structure, biochemistry, and possibly function that has occurred in the brainstem, and especially in the phylogenetically old reticular formation. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 01:26:35