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Titolo:
Genetic control of neuroadapted Sindbis virus replication in female mice maps to chromosome 2 and associates with paralysis and mortality
Autore:
Thach, DC; Kleeberger, SR; Tucker, PC; Griffin, DE;
Indirizzi:
Johns Hopkins Univ, Bloomberg Sch Publ Hlth, W Harry Feinstone Dept Mol Microbiol & Immunol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MDUSA 21205 mmunol, Baltimore, MD 21205 USA Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Environm Hlth Sci, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 th Sci, Baltimore, MD 21205 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 18, volume: 75, anno: 2001,
pagine: 8674 - 8680
SICI:
0022-538X(200109)75:18<8674:GCONSV>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRACTIONATED X-IRRADIATION; CENTRAL-NERVOUS-SYSTEM; HOST-RESISTANCE LOCUS; THEILERS-VIRUS; MOUSE CHROMOSOME-6; PERSISTENT INFECTION; RETROVIRAL INFECTION; LETHAL INFECTION; ECTROMELIA VIRUS; FV-4 RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Griffin, DE Johns Hopkins Univ, Bloomberg Sch Publ Hlth, W Harry FeinstoneDept Mol Microbiol & Immunol, 615 N Wolfe St, Baltimore, MD 21205 USA Johns Hopkins Univ 615 N Wolfe St Baltimore MD USA 21205 5 USA
Citazione:
D.C. Thach et al., "Genetic control of neuroadapted Sindbis virus replication in female mice maps to chromosome 2 and associates with paralysis and mortality", J VIROLOGY, 75(18), 2001, pp. 8674-8680

Abstract

Neuroadapted Sindbis virus (NSV) infection of mice causes hindlimb paralysis and 100% mortality in the C57BL/6 mouse strain, while adults of the BALB/cBy mouse strain are resistant to fatal encephalomyelitis. Levels of viralRNA are higher in the brains of infected C57BL/6 mice than in BALB/cBy mice (D. C. Thach et al., J. Virol. 74:6156-6161, 2000). These phenotypic differences between the two strains allowed us to map genetic loci involved in mouse susceptibility to NSV and to find relationships between mortality, paralysis, and viral RNA levels. Analysis of percent mortality in H2-congenicand F-1 mice suggested that the H2 locus, sex linkage, and imprinting werenot involved in determining susceptibility and that resistance was partially dominant over susceptibility. Segregation analysis using CXB recombinantinbred (RI) mice indicated that the percent mortality was multigenic. Interval mapping detected a suggestive quantitative trait locus (QTL) on chromosome 2 near marker D2Mit447. Analysis of paralysis in the RI mice detected the same suggestive QTL. Viral RNA level in F-1 mice was intermediate. Interval mapping using viral RNA levels in RI mice detected a significant QTL near marker D2Mit447 that explained 69% of the genetic variance. This QTL was confirmed in F2 mice and was designated as Nsv1. Viral RNA level, percentparalyzed, and percent mortality were linearly correlated (r = 0.8 to 0.9). These results indicate that mortality, paralysis, and viral RNA levels are related complex traits and that Nsv1 controls early viral load and determines the likelihood of paralysis and death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 10:06:44