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Titolo:
Identification of the immunodominant H-2K(k)-restricted cytotoxic T-cell epitope in the Borna disease virus nucleoprotein
Autore:
Schamel, K; Staeheli, P; Hausmann, J;
Indirizzi:
Univ Freiburg, Dept Virol, Abt Virol, Inst Med Mikrobiol & Hyg, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 & Hyg, D-79104 Freiburg, Germany
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 18, volume: 75, anno: 2001,
pagine: 8579 - 8588
SICI:
0022-538X(200109)75:18<8579:IOTIHC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MHC CLASS-I; NATURALLY PROCESSED PEPTIDES; HEPATITIS-C VIRUS; INFECTED RATS; LYMPHOCYTES; ANTIGEN; BRAIN; ENCEPHALITIS; EXPRESSION; RESPONSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Hausmann, J Univ Freiburg, Dept Virol, Abt Virol, Inst Med Mikrobiol & Hyg, Hermann Herder Str 11, D-79104 Freiburg, Germany Univ Freiburg Hermann Herder Str 11 Freiburg Germany D-79104
Citazione:
K. Schamel et al., "Identification of the immunodominant H-2K(k)-restricted cytotoxic T-cell epitope in the Borna disease virus nucleoprotein", J VIROLOGY, 75(18), 2001, pp. 8579-8588

Abstract

Borna disease virus (BDV)-induced immunopathology in mice is most prominent in strains carrying the major histocompatibility complex H-2k allele and is mediated by CD8(+) T cells that are directed against the viral nucleoprotein p40. We now identified the highly conserved octamer peptide TELEISSI, located between amino acid residues 129 and 136 of BDV p40, as a potent H-2K(k)-restricted cytotoxic T-cell (CTL) epitope. When added to the culture medium of L929 target cells, TELEISSI conferred sensitivity to lysis by CTLsisolated from brains of BDV-infected MRL mice with acute neurological disease. Vaccinia virus-mediated expression of a p40 variant with mutations in the two K-k-specific anchor residues of the TELEISSI peptide (p40(E130K,I136T)) did not sensitize L929 target cells for lysis by BDV-specific CTLs, whereas expression of wild-type p40 did. Furthermore, unlike vaccination withwild-type p40, vaccination of persistently infected symptomless B10.BR mice with p40(E130K,I136T) did not result in central nervous system inflammation and neurological disease. These results demonstrate that TELEISSI is theimmunodominant CTL epitope of BDV p40 in H-2k mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:35:58