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Titolo:
Expression and function of chemokine receptors on human thymocytes: Implications for infection by human immunodeficiency virus type 1
Autore:
Taylor, JR; Kimbrell, KC; Scoggins, R; Delaney, M; Wu, LJ; Camerini, D;
Indirizzi:
Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Myles H Thaler Ctr AIDS & Human Retrovirus Res, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Millennium Pharmaceut, Cambridge, MA 02139 USA Millennium Pharmaceut Cambridge MA USA 02139 eut, Cambridge, MA 02139 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 18, volume: 75, anno: 2001,
pagine: 8752 - 8760
SICI:
0022-538X(200109)75:18<8752:EAFOCR>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCID-HU MOUSE; DISEASE PROGRESSION; ACQUIRED IMMUNODEFICIENCY; REPLICATION KINETICS; HIV-1 TRANSMISSION; CORECEPTORS CXCR4; DISTINCT SUBSETS; LYMPHOID ORGANS; AMINO-TERMINUS; T-LYMPHOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Camerini, D Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 ille, VA 22908 USA
Citazione:
J.R. Taylor et al., "Expression and function of chemokine receptors on human thymocytes: Implications for infection by human immunodeficiency virus type 1", J VIROLOGY, 75(18), 2001, pp. 8752-8760

Abstract

The presence or absence of the receptor CD4 and the coreceptors CCR5 and CXCR4 restrict the cell tropism of human immunodeficiency virus type 1 (HIV-1). Despite the importance of thymic infection by HIV-1, conflicting reports regarding the expression of HIV-1 coreceptors on human thymocytes have not been resolved. We assayed the expression and function of the major HIV-1 coreceptors, CCR5 and CXCR4, as well as CCR4 and CCR7 as controls, on humanthymocytes. We detected CCR5 on 2.5% of thymocytes, CXCR4 on 53% of the cells, and CCR4 on 16% and CCR7 on 11% of human thymocytes. Moreover, infection by R5 HIV-1 did not significantly induce expression of CCR5. We found that two widely used anti-CCR5 monoclonal antibodies cross-reacted with CCR8,which may account for discrepancies among published reports of CCR5 expression on primary cells. This cross-reactivity could be eliminated by deletion of amino acids 2 through 4 of CCR8. Chemotaxis assays showed that SDF-1, which binds CXCR4; MDC, which binds CCR4; and ELC, which binds CCR7, mediated significant chemotaxis of thymocytes. In contrast, MIP-1 beta, whose receptor is CCR5, did not induce significant chemotaxis. Our results indicate that CXCR4, CCR4, CCR7, and their chemokine ligands may be involved in thymocyte migration during development in the thymus. CCR5 and its ligands, however, are likely not involved in these processes. Furthermore, the pattern of CCR5 and CXCR4 expression that we found may explain the greater susceptibility of human thymocytes to infection by HIV-1 isolates capable of using CXCR4 in cell entry compared to those that use only CCR5.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 11:58:55