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Titolo:
Response of intestinal proteinase activities to the feeding of isolated winged bean (Psophocarpus tetragonolobus) and soya bean (Glycine max) trypsininhibitors in rats
Autore:
Nishino, N; Taniguchi, M; Sakaguchi, E;
Indirizzi:
Okayama Univ, Fac Agr, Dept Anim Sci, Okayama 7008530, Japan Okayama UnivOkayama Japan 7008530 Dept Anim Sci, Okayama 7008530, Japan
Titolo Testata:
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
fascicolo: 11, volume: 81, anno: 2001,
pagine: 1055 - 1059
SICI:
0022-5142(20010901)81:11<1055:ROIPAT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC-ENZYME-SECRETION; FEEDBACK-REGULATION; SOYBEANS; LECTIN;
Keywords:
soya bean; trypsin inhibitors; winged bean;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Nishino, N Okayama Univ, Fac Agr, Dept Anim Sci, 2-5-1 Shikata Cho, Okayama 7008530, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008530 30, Japan
Citazione:
N. Nishino et al., "Response of intestinal proteinase activities to the feeding of isolated winged bean (Psophocarpus tetragonolobus) and soya bean (Glycine max) trypsininhibitors in rats", J SCI FOOD, 81(11), 2001, pp. 1055-1059

Abstract

The antinutritional activities of trypsin inhibitors (TIs) were compared between winged beans (Psophocarpus tetragonolobus) and soya beans (Glycine max). The inhibitors of the two beans were isolated by trypsin-bound Sepharose 4B, and 50 mg of lyophilised powders were intubated intragastrically into 24 h fasted rats. The activities of trypsin and chymotrypsin were compared after 30, 60 and 180 min in the washings of the upper, middle and lower parts of the small intestine. The elution profiles of TI and non-TI compounds in the affinity chromatography were similar in the two beans, and the antitryptic activities were concentrated 5.5 and 6.2 times (based on specific activity) for winged beans and soya beans respectively. Regardless of the TI fed to rats, trypsin activity in the upper intestine was suppressed to almost undetectable levels at 30 and 60 min after intubation. The activities in the middle and lower intestines were also substantially lowered when rats were fed winged bean TI, and significant differences were detected at 30 and 60 min after intubation when compared with rats fed soya bean TI. However, at ISO min after feeding, no differences were found in the trypsin activity in any gut segments. Similar inhibitory properties of isolated TIs were observed in chymotrypsin activities in the small intestine. The results suggest that winged bean TI may have greater inhibitory activity on the intestinal proteinase compared with soya bean TI. (C) 2001 Society of Chemical Industry.

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Documento generato il 22/10/20 alle ore 09:02:12