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Titolo:
In vivo use of oligonucleotides to inhibit choroidal neovascularisation inthe eye
Autore:
Garrett, KL; Shen, WY; Rakoczy, PE;
Indirizzi:
Lions Eye Inst, Dept Mol Ophthalmol, Nedlands, WA 6009, Australia Lions Eye Inst Nedlands WA Australia 6009 l, Nedlands, WA 6009, Australia Univ Western Australia, Ctr Ophthalmol & Visual Sci, Nedlands, WA 6009, Australia Univ Western Australia Nedlands WA Australia 6009 nds, WA 6009, Australia
Titolo Testata:
JOURNAL OF GENE MEDICINE
fascicolo: 4, volume: 3, anno: 2001,
pagine: 373 - 383
SICI:
1099-498X(200107/08)3:4<373:IVUOOT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; INTERNAL RIBOSOME ENTRY; FACTOR MESSENGER-RNA; PHOSPHOROTHIOATE OLIGONUCLEOTIDES; SUBRETINAL NEOVASCULARIZATION; RETINAL NEOVASCULARIZATION; MACULAR DEGENERATION; FACTOR EXPRESSION; UP-REGULATION; FACTOR VEGF;
Keywords:
VEGF; choroidal neovascularisation; oligonucleotides; eye;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Rakoczy, PE Lions Eye Inst, Dept Mol Ophthalmol, 2 Verdun St, Nedlands, WA6009, Australia Lions Eye Inst 2 Verdun St Nedlands WA Australia 6009 ustralia
Citazione:
K.L. Garrett et al., "In vivo use of oligonucleotides to inhibit choroidal neovascularisation inthe eye", J GENE MED, 3(4), 2001, pp. 373-383

Abstract

Background We have previously demonstrated the in vivo uptake of oligonucleotides in the rat eye and have continued with experiments to look at the effectiveness of targeted oligonucleotide sequences. Vascular endothelial growth factor (VEGF) is correlated with new blood vessel formation and has been implicated in numerous eye diseases characterised by abnormal blood vessel proliferation. An oligonucleotide targeted to the VEGF sequence was examined for its effect on VEGF production in vitro and the development of choroidal neovascularisation in vivo in the eye. Methods A series of sequences were assessed in an in vitro screening system using retinal pigment epithelial (RPE) cells to demonstrate a reduction in VEGF. A targeted sequence was further investigated using an animal model of choroidal neovascularisation where a krypton laser was used to produce awound healing response in the choroid and retina. The oligonucleotide was injected into the vitreous and the development of choroidal neovascularisation assessed using fluorescein angiography. Results The targeted sequence was shown in vitro to downregulate the VEGF produced by RPE cells grown under hypoxic conditions and when injected intolaser treated eyes was shown to be preferentially taken up in the laser lesion. Fluorescein angiography demonstrated that the test oligonucleotide was successful in reducing laser-mediated choroidal neovascularisation. Conclusions A sequence corresponding to the 5'UTR of the VEGF gene has provided encouraging results for the treatment of neovascularisation. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 16:06:49