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Titolo:
Combined transductional and transcriptional targeting of melanoma cells byartificial virus-like particles
Autore:
Nahde, T; Muller, K; Fahr, A; Muller, R; Brusselbach, S;
Indirizzi:
Univ Marburg, Inst Mol Biol & Tumor Res, D-35033 Marburg, Germany Univ Marburg Marburg Germany D-35033 Tumor Res, D-35033 Marburg, Germany Univ Marburg, Inst Pharmaceut Technol & Biopharmaceut, D-35032 Marburg, Germany Univ Marburg Marburg Germany D-35032 harmaceut, D-35032 Marburg, Germany Vectron Therapeut AG, D-35037 Marburg, Germany Vectron Therapeut AG Marburg Germany D-35037 G, D-35037 Marburg, Germany
Titolo Testata:
JOURNAL OF GENE MEDICINE
fascicolo: 4, volume: 3, anno: 2001,
pagine: 353 - 361
SICI:
1099-498X(200107/08)3:4<353:CTATTO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-V INTEGRINS; GENE-TRANSFER; IN-VIVO; POLY(ETHYLENIMINE)/DNA COMPLEXES; ENDOTHELIAL-CELLS; NONVIRAL VECTOR; BETA-3 INTEGRIN; EXPRESSION; DELIVERY; ADENOVIRUS;
Keywords:
non-viral vectors; artificial virus-like envelope (AVE); artificial virus-like particle (AVP); polyethyleneimine; melanoma cells; integrin targeting; cyclic RGD; transductional targeting; transcriptional targeting; tyrosinase promoter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Muller, K Univ Marburg, Inst Mol Biol & Tumor Res, Emil Mannkopf Str 2, D-35033 Marburg, Germany Univ Marburg Emil Mannkopf Str 2 Marburg Germany D-35033 ermany
Citazione:
T. Nahde et al., "Combined transductional and transcriptional targeting of melanoma cells byartificial virus-like particles", J GENE MED, 3(4), 2001, pp. 353-361

Abstract

Background Artificial virus-like particles (AVPs) represent a novel type of liposomal vector resembling retroviral envelopes. AVPs are serum-resistant and non-toxic and can be endowed with a peptide ligand as a targeting device. The vitronectin receptor, alpha (v)beta (3)-integrin, is commonly upregulated on malignant melanoma cells. in the present study we investigated whether AVPs carrying cyclic peptides with an RGD integrin binding motif (RGD-AVPs) are suitable for the specific and efficient transduction of human melanoma cells. Methods Plasmid DNA was complexed with low molecular weight non-linear polyethyleneimine and packaged into anionic liposomes. Transduction efficiencies were determined after transient transfection of different cell lines in serum-free medium using green fluorescent protein or luciferase reporter genes. Results We demonstrated that RGD-AVPs transduced human melanoma cells withhigh efficiencies of > 60%. Efficient transduction was clearly dependent on the presence of the cyclic RGD ligand and was selective for melanoma cells. The specificity of the vector system could be further enhanced by using the melanocyte-specific tyrosinase promoter to drive transgene expression. Conclusion Our findings suggest that the AVP technology is a useful approach for generating highly efficient and specific non-viral vectors for melanoma targeting, in particular in a setting of combined transductional and transcriptional targeting. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 03:34:34