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Titolo:
Efficiency of liposomes surface-modified with soybean-derived sterylglucoside as a liver targeting carrier in HepG2 cells
Autore:
Maitani, Y; Kawano, K; Yamada, K; Nagai, T; Takayama, K;
Indirizzi:
Hoshi Univ, Dept Pharmaceut, Shinagawa Ku, Tokyo 1428501, Japan Hoshi Univ Tokyo Japan 1428501 aceut, Shinagawa Ku, Tokyo 1428501, Japan Fujita Hlth Univ, Inst Comprehens Med Sci, Toyoake, Aichi 4701192, Japan Fujita Hlth Univ Toyoake Aichi Japan 4701192 oyoake, Aichi 4701192, Japan
Titolo Testata:
JOURNAL OF CONTROLLED RELEASE
fascicolo: 3, volume: 75, anno: 2001,
pagine: 381 - 389
SICI:
0168-3659(20010810)75:3<381:EOLSWS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-GLUCOSYL-NEOGLYCOPROTEINS; DIPALMITOYLPHOSPHATIDYLCHOLINE LIPOSOMES; ASIALOGLYCOPROTEIN RECEPTOR; GALACTOSYLATED ALBUMIN; DOXORUBICIN; ASIALOFETUIN; MIXTURE; INVIVO; RAT; PHARMACOKINETICS;
Keywords:
liposome; soybean-derived sterylglucoside; asialoglycoprotein receptor; HepG2 cells; liver targeting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Maitani, Y Hoshi Univ, Dept Pharmaceut, Shinagawa Ku, Ebara 2-4-41, Tokyo 1428501, Japan Hoshi Univ Ebara 2-4-41 Tokyo Japan 1428501 kyo 1428501, Japan
Citazione:
Y. Maitani et al., "Efficiency of liposomes surface-modified with soybean-derived sterylglucoside as a liver targeting carrier in HepG2 cells", J CONTR REL, 75(3), 2001, pp. 381-389

Abstract

We investigated the interaction of liposomes surface-modified with soybean-derived sterylglucoside (SG) (SG-liposomes) with HepG2 cells in the point of involvement of asialoglycoprotein receptor (ASGP-R) mediated endocytosisand examined the efficiency of SG-liposomcs as drug carriers using 1,1 ' -dioctadccyl-3,3,3 ' ,3 ' -tetramethylindocarbocyanine perchlorate (Dil) as a maker of liposome, carboxylated polystyrene microspheres (Fluoresbrite) as a model drug not taken up in cells and doxorubicin (DXR). SG-liposomes were composed of dipalmitoylphosphatidylcholine (DPPC), cholesterol (Ch) and SG (DPPC/Ch/SG=6:3:1, molar ratio) and Dil, Fluoresbrite and DXR were entrapped in SG-liposomes, respectively. Each SG-liposome was incubated with HepG2 cells at 4 or 37 degreesC, and co-incubated with asialofetuin (AF) as a competitor of ASGP-R. The association of Dil, Fluoresbrite or DXR entrappedin SG-liposomes with HepG2 cells at 37 degreesC was significantly higher than that in liposomes containing no SG. That of Dil and Fluoresbrite was reduced significantly by the incubation with AF, but that of DXR was not affected. These findings suggest that Fluoresbrite behaves like the lipid component of SG-liposomes, but DXR in SG-liposomes does not behave similar to the lipid component of SG-liposomes, thus, its drug behavior released from liposomes may be due to its physicochemical properties. SG-liposomes are potentially useful drug carriers to the liver, because the glucose residue may work as a kind of ligand for ASGP-R. (C) 2001 Elsevier Science BY All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 05:55:50