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Titolo:
Analysis of lycopene geometrical isomers in biological microsamples by liquid chromatography with coulometric array detection
Autore:
Ferruzzi, MG; Nguyen, ML; Sander, LC; Rock, CL; Schwartz, SJ;
Indirizzi:
Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 i & Technol, Columbus, OH 43210 USA NIST, Chem Sci & Technol Lab, Div Analyt Chem, Gaithersburg, MD 20899 USA NIST Gaithersburg MD USA 20899 iv Analyt Chem, Gaithersburg, MD 20899 USA Univ Calif San Diego, Dept Family & Prevent Med, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 nt Med, La Jolla, CA 92093 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 760, anno: 2001,
pagine: 289 - 299
SICI:
1387-2273(20010905)760:2<289:AOLGII>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-CAROTENE; HUMAN PLASMA; ALPHA-TOCOPHEROL; CERVICAL TISSUE; TRANS-LYCOPENE; RETINOL; CANCER; SEPARATION; PROSTATE;
Keywords:
geometrical isomers; coulometric array detection; lycopene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Schwartz, SJ Ohio State Univ, Dept Food Sci & Technol, 2001 Fyffe Court, Columbus, OH 43210 USA Ohio State Univ 2001 Fyffe Court Columbus OH USA 43210 10 USA
Citazione:
M.G. Ferruzzi et al., "Analysis of lycopene geometrical isomers in biological microsamples by liquid chromatography with coulometric array detection", J CHROMAT B, 760(2), 2001, pp. 289-299

Abstract

Methods of analysis for determining low quantities of lycopene cis-trans isomers in biological tissues are needed. Development of two liquid chromatography (LC) methods based on the polymeric C-30 stationary phase equipped with coulometric electrochemical array detection (ED) is described. Separation of 13 lycopene isomers including prolycopene, (a novel tetra-cis-lycopene found in Tangerine tomatoes) was accomplished with both isocratic and gradient methods using different proportions of methanol, methyl tert.-butyl ether, water and 1 M ammonium acetate buffer. Carotenoids were detected at potential settings between 200 and 620 mV. Differences in generated current-voltage curves aided in tentative identification of trans carotenoid species and select cis isomers of lycopene. These methods were successfully applied in the analysis of small quantities of plasma, buccal mucosal cells, prostate and cervical tissues. Limits of detection for trans-lycopene by ED were found to be 50 fmol representing a 10- to 100-fold increase over conventional UV-Vis absorbance methods. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:19:39