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Titolo:
Development of a simplified, sensitive high-performance liquid chromatographic method using fluorescence detection to determine the concentration of UCN-01 in human plasma
Autore:
Smith, JA; Cortes, J; Newman, RA; Madden, TL;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Div Pharm, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 anc Ctr, Div Pharm, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Div Canc Med, Houston, TX 77030 USA UnivTexas Houston TX USA 77030 Ctr, Div Canc Med, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Pharmaceut Dev Ctr, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 Pharmaceut Dev Ctr, Houston, TX 77030 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 760, anno: 2001,
pagine: 247 - 253
SICI:
1387-2273(20010905)760:2<247:DOASSH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; CLINICAL-PHARMACOLOGY; SELECTIVE INHIBITOR; CANCER-CELLS; 7-HYDROXYSTAUROSPORINE; CHECKPOINT; APOPTOSIS; CYTOTOXICITY; CAMPTOTHECIN; ACTIVATION;
Keywords:
UCN-01;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Madden, TL Univ Texas, MD Anderson Canc Ctr, Div Pharm, 1515 Holcombe Blvd,Box 90, Houston, TX 77030 USA Univ Texas 1515 Holcombe Blvd,Box 90 HoustonTX USA 77030 0 USA
Citazione:
J.A. Smith et al., "Development of a simplified, sensitive high-performance liquid chromatographic method using fluorescence detection to determine the concentration of UCN-01 in human plasma", J CHROMAT B, 760(2), 2001, pp. 247-253

Abstract

UCN-01 is a naturally derived anticancer agent isolated in the culture broth of actinomyces streptomyces. We have developed a sensitive high-performance liquid chromatographic method for the determination of UCN-01 in human plasma. UCN-01 was isolated from human plasma after intravenous administration, by using 100% ice-cold acetonitrile liquid-liquid phase extraction. Liquid chromatographic separation was achieved by isocratic elution on a phenyl analytical column. The mobile phase consisted of acetonitrile-0.5 M ammonium acetate (45:55) with 0.2% triethylamine added as a modifier. The UCN-01 peak was identified from other peaks using fluorescence excitation energyand emission energy wavelengths of 310 and 410 nm, respectively. Retentiontime for UCN-01 was 4.2 +/-0.5 min. The UCN-01 peak was baseline resolved,with nearest peak at 2.6 min distance. No interfering peaks were observed at the retention time of UCN-01. Peak area amounts from extracted samples were proportional over the dynamic concentration range used: 0.2 to 30 mug/ml. Mean recoveries of UCN-01 at concentrations of 0.5 and 25 mug/ml were 89and 90.2%, respectively. Relative standard deviations for UCN-01 calibration standards ranged from 1.89 to 2.31%, with relative errors ranging from 0.3 to 11.6%. Assay precision for UCN-01 based on quality control samples of0.50 mug/ml was +/-4.86% with an accuracy of +/-5.7%. For drug extracted from plasma the lowest limit of detection was 0.1 mug/ml, with the lowest limit of quantitation being 0.2 mug/ml. This method is suitable for routine analysis of UCN-01 in human plasma at concentration from 0.2 to 30 mug/ml. (C) 2001 Elsevier Science BV All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:43:26