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Titolo:
Quantitative analysis of, the principle soy isoflavones genistein, daidzein and glycitein, and their primary conjugated metabolites in human plasma and urine using reversed-phase high-performance liquid chromatography with ultraviolet detection
Autore:
Thomas, BF; Zeisel, SH; Busby, MG; Hill, JM; Mitchell, RA; Scheffler, NM; Brown, SS; Bloeden, LT; Dix, KJ; Jeffcoat, AR;
Indirizzi:
Res Triangle Inst, Res Triangle Pk, NC 27709 USA Res Triangle Inst Res Triangle Pk NC USA 27709 Triangle Pk, NC 27709 USA Univ N Carolina, Sch Med, Sch Publ Hlth, Dept Nutr,CB 7400, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 7400, Chapel Hill, NC 27599 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 760, anno: 2001,
pagine: 191 - 205
SICI:
1387-2273(20010905)760:2<191:QAOTPS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
IONIZATION MASS-SPECTROMETRY; DIETARY PHYTOESTROGENS; CAPILLARY ELECTROPHORESIS; VEGETARIAN WOMEN; PHYTO-ESTROGENS; ARRAY DETECTION; HPLC ANALYSIS; LIGNANS; RADIOIMMUNOASSAY; COUMESTROL;
Keywords:
genistein; daidzein; glycitein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Thomas, BF Res Triangle Inst, 3040 Cornwallis Rd, Res Triangle Pk, NC 27709 USA Res Triangle Inst 3040 Cornwallis Rd Res Triangle Pk NC USA 27709
Citazione:
B.F. Thomas et al., "Quantitative analysis of, the principle soy isoflavones genistein, daidzein and glycitein, and their primary conjugated metabolites in human plasma and urine using reversed-phase high-performance liquid chromatography with ultraviolet detection", J CHROMAT B, 760(2), 2001, pp. 191-205

Abstract

Soy isoflavones are becoming of increasing interest as nutritional agents which can be used to combat osteoporosis and hyperlipidemia, and are also being considered as potential cancer chemopreventive compounds. However, prior to their formulation and distribution as therapeutic agents, thorough pharmacokinetic and toxicological assessment needs to be completed in men andwomen in a variety of health conditions in order to ensure their therapeutic efficacy and safety. At this time, studies of purified soy isoflavones are possible, and are being designed to fully evaluate the pharmacological utility of these preparations. In support of these studies, quantitative analysis of soy isoflavones in biological fluids can be accomplished with a wide variety of methods and analytical instrumentation. However, the relatively ubiquitous presence of high-performance liquid chromatography with ultraviolet detection (HPLC-UV) in most analytical laboratories, the relative ease of its operation, and the lesser expense of this instrumentation as compared to more sophisticated techniques such as liquid chromatography-mass spectrometry, offers some distinct advantages for its use in pharmacokinetic studies. In this manuscript, the development and validation of an HPLC-UV method for the quantitation of the prinicipal soy isoflavones,,genistein, daidzein, and glycitein, and their primary metabolites, in human plasma and urine is described. This analytical approach allows for pharmacologically relevant concentrations of the analytes and their principle metabolites to bedetected, and has been validated in close agreement with the US Food and Drug Administration's guidelines for the validation of methods to be used insupport of pharmacokinetic studies. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 12:50:16