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Titolo:
Human DNA polymerase iota promiscuous mismatch extension
Autore:
Vaisman, A; Tissier, A; Frank, EG; Goodman, MF; Woodgate, R;
Indirizzi:
NICHHD, Sect DNA Replicat Repair & Mutagenesis, NIH, Bethesda, MD 20892 USA NICHHD Bethesda MD USA 20892 r & Mutagenesis, NIH, Bethesda, MD 20892 USA Univ So Calif, Dept Sci Biol, Hedco Mol Biol Labs, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 ol Labs, Los Angeles, CA 90089 USA Univ So Calif, Dept Chem, Hedco Mol Biol Labs, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 ol Labs, Los Angeles, CA 90089 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 33, volume: 276, anno: 2001,
pagine: 30615 - 30622
SICI:
0021-9258(20010817)276:33<30615:HDPIPM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
XERODERMA-PIGMENTOSUM; GENE ENCODES; THYMINE DIMER; FIDELITY; BYPASS; REPLICATION; ETA; MUTAGENESIS; PROTEIN; MISINSERTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Woodgate, R NICHHD, Sect DNA Replicat Repair & Mutagenesis, NIH, Bldg 6,Rm1A13,9000 Rockville Pike, Bethesda, MD 20892 USA NICHHD Bldg 6,Rm 1A13,9000 Rockville Pike Bethesda MD USA 20892
Citazione:
A. Vaisman et al., "Human DNA polymerase iota promiscuous mismatch extension", J BIOL CHEM, 276(33), 2001, pp. 30615-30622

Abstract

Human DNA polymerase iota is a low-fidelity template copier that preferentially catalyzes the incorporation of the wobble base G, rather than the Watson-Crick base A, opposite template T (Tissier, A., McDonald, CF. P., Frank, E. G., and Woodgate, R. (2000) Genes Dev. 14, 1642-1650; Johnson, R. E., Washington, M. T., Haracska, L., Prakash, S., and Prakash, L. (2000) Nature406, 10151019; Zhang, Y., Yuan, F., Wu, X., and Wang, Z. (2000) Mol. Cell. Biol. 20,7099-7108). Here, we report on its ability to extend all 12 possible mispairs and 4 correct pairs in different sequence contexts. Extension from both matched and mismatched primer termini is generally most efficientand accurate when A is the next template base. In contrast, extension occurs less efficiently and accurately when T is the target template base. A striking exception occurs during extension of a G:T mispair, where the enzymeswitches specificity, "preferring" to make a correct A.T base pair immediately downstream from an originally favored G:T mispair. Polymerase L generates a variety of single and tandem mispairs with high frequency, implying that it may act as a strong mutator when copying undamaged DNA templates in vivo. Even so, its limited ability to catalyze extension from a relatively stable primer/template containing a "buried" mismatch suggests that polymerase iota -catalyzed errors are confined to short template regions.

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Documento generato il 09/04/20 alle ore 00:27:02