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Titolo:
Expression and putative role of 11 beta-hydroxysteroid dehydrogenase isozymes within the human eye
Autore:
Rauz, S; Walker, EA; Shackleton, CHL; Hewison, M; Murray, PI; Stewart, PM;
Indirizzi:
Childrens Hosp, Oakland Res Inst, Mass Spectrometry Facil, Oakland, CA 94609 USA Childrens Hosp Oakland CA USA 94609 trometry Facil, Oakland, CA 94609 USA Univ Birmingham, Dept Endocrinol, Div Med Sci, Birmingham, W Midlands, England Univ Birmingham Birmingham W Midlands England ngham, W Midlands, England Univ Birmingham, Div Immun & Infect, Acad Unit Ophthalmol, Birmingham, W Midlands, England Univ Birmingham Birmingham W Midlands England ngham, W Midlands, England
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 9, volume: 42, anno: 2001,
pagine: 2037 - 2042
SICI:
0146-0404(200108)42:9<2037:EAPRO1>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
APPARENT MINERALOCORTICOID EXCESS; INTRAOCULAR-PRESSURE; AQUEOUS-HUMOR; TISSUE DISTRIBUTION; CILIARY BODY; LOCALIZATION; GLAUCOMA; TYPE-2; GENE; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Stewart, PM Univ Birmingham, Queen Elizabeth Hosp, Div Med Sci, BirminghamB15 2TH, W Midlands, England Univ Birmingham Birmingham W Midlands England B15 2TH England
Citazione:
S. Rauz et al., "Expression and putative role of 11 beta-hydroxysteroid dehydrogenase isozymes within the human eye", INV OPHTH V, 42(9), 2001, pp. 2037-2042

Abstract

PURPOSE. The human eye is an important target tissue for steroid hormones,and glucocorticoids have been implicated in the pathogenesis of ocular disease, including glaucoma. In peripheral tissues, corticosteroid hormone action is regulated at a prereceptor level through the activity of the 11 beta-hydroxy-steroid dehydrogenase (11 beta -HSD) isozymes: an oxo-reductase (11 beta -HSD1) that activates cortisol (F) from cortisone (E) and a dehydrogenase (11 beta -HSD2) that inactivates F to E. The purpose of this study was to analyze the expression and putative role of 11 beta -HSD within the human eye. METHODS. Immunohistochemical and reverse transcription-polymerase chain reaction (RT-PCR) studies were performed on sections of human ocular tissues,surgical trabecular meshwork (TM) specimens and a ciliary nonpigmented epithelial (NPE) cell-line. Free F and E concentrations in aqueous humor were determined by gas chromatography-mass spectrometry (GC/MS). IOP was measured in eight male volunteers before and after oral ingestion of carbenoxolone(CBX), a known inhibitor of 11 beta -HSD. RESULTS. 11 beta -HSD1 was expressed in the basal cells of the corneal epithelium and the NPE. 11 beta -HSD2 was restricted to the corneal endothelium. RT-PCR revealed mRNA for only the glucocorticoid receptor (GR) in the TMspecimens, whereas GR, mineralocorticoid receptor and 11 beta -HSD1 mRNAs were all present in the NPE cell line. The demonstration of free F in excess of E (F/E 14:1) in the aqueous humor suggested predominant 11 beta -HSD1 activity. Compared with baseline (14.7 +/- 1.06 mm Hg, mean +/- SD), the IOP decreased significantly on both the third and seventh days of CBX ingestion (12.48 +/- 1.11 mm Hg, P < 0.0001 and 11.78 +/- 1.50 min Hg, P < 0.0001,respectively). CONCLUSIONS. These results suggest that the 11 beta -HSD1 isozyme may modulate steroid-regulated sodium transport across the NPE, thereby influencingIOP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/07/18 alle ore 23:58:44