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Titolo:
A novel role of tissue factor pathway inhibitor-2 in apoptosis of malignant human gliomas
Autore:
Tasiou, A; Konduri, SD; Yanamandra, N; Dinh, DH; Olivero, WC; Gujrati, M; Obeyesekere, M; Rao, JS;
Indirizzi:
Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 ci, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois, Coll Med, Dept Neurosurg, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 d, Dept Neurosurg, Peoria, IL 61656 USA Univ Illinois, Coll Med, Dept Neuropathol, Div Canc Biol, Peoria, IL 61656USA Univ Illinois Peoria IL USA 61656 hol, Div Canc Biol, Peoria, IL 61656USA Univ Texas, MD Anderson Canc Ctr, Dept Biomath, Houston, TX 77030 USA UnivTexas Houston TX USA 77030 Ctr, Dept Biomath, Houston, TX 77030 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 3, volume: 19, anno: 2001,
pagine: 591 - 597
SICI:
1019-6439(200109)19:3<591:ANROTF>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERINE PROTEINASE-INHIBITORS; FAS-MEDIATED APOPTOSIS; CYTOCHROME-C; MESSENGER-RNA; GENE-PRODUCT; BCL-2; CELLS; EXPRESSION; CASPASES; KINASE;
Keywords:
TFPI-2; apoptosis; glioblastoma; caspases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Rao, JS Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, 1 Illini Dr, Peoria, IL 61656 USA Univ Illinois 1 Illini Dr Peoria IL USA 61656 Peoria, IL 61656 USA
Citazione:
A. Tasiou et al., "A novel role of tissue factor pathway inhibitor-2 in apoptosis of malignant human gliomas", INT J ONCOL, 19(3), 2001, pp. 591-597

Abstract

Tissue factor pathway inhibitor-2 (TFPI-2) is a 32 kDa serine protease inhibitor found at high levels in extracellular matrix. Recombinant human TFPI-2 has recently been shown to be a strong inhibitor of trypsin, plasmin, plasma kallikrein, and factor XIa amidolytic activity. Earlier studies in ourlaboratory showed that the expression of TFPI-2 is lost during tumor progression in human gliomas. We stably transfected this protease inhibitor in multiform glioblastoma cell line (SN13-19) and in low-grade glioma cell line(Hs683) in sense and antisense orientation respectively. This confirmed that the upregulation/down-regulation of TFPI-2 plays a significant role in the invasive behavior of human gliomas both in vitro and in vivo models. Collectively, these results suggested an idea to determine whether TFPI-2 is necessary for cell survival and inhibition of tumor formation in nude mice, due to apoptosis of intracerebrally injected SNB-19 cells. In the present study we determined p-ERK levels and found that they are decreased in TFPI-2overexpressed clones (SNB-19) and increased in TFPI-2 downregulated clones(Hs683). We also checked the levels of BAX/BCl-2, caspases (for e.g., 9, 7, 3, 8), PARP, cytochrome-c and Apaf-1. Moreover, the increase of apoptosisin vitro is associated with increased and decreased expression of apoptotic protein BAX in sense clones (SNB-19) and antisense clones (Hs683) respectively, when compared to controls and vice versa with Bcl-2 the anti-apoptotic protein. Caspases (9, 7 and 3), cytochrome-c, Apaf-1 and PARP levels areincreased in SNB-19 and decreased in Hs683. Caspase 8 was not expressed ineither cell line. Caspases 9 and 3 activity assay revealed higher activityin sense clones (SNB-19) but lesser in antisense clones (Hs683) compared to controls. This is the first report of TFPI-2 playing a novel role in cellsurvival in human gliomas.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 07:32:26