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Titolo:
Inhibition of endive (Cichorium endivia L.) polyphenoloxidase by a Carica papaya latex preparation
Autore:
de Rigal, D; Cerny, M; Richard-Forget, F; Varoquaux, P;
Indirizzi:
INRA, UBTAR, F-33883 Villenave Dornon, France INRA Villenave Dornon France F-33883 R, F-33883 Villenave Dornon, France INRA, Stn Technol, F-84914 Avignon, France INRA Avignon France F-84914INRA, Stn Technol, F-84914 Avignon, France
Titolo Testata:
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
fascicolo: 6, volume: 36, anno: 2001,
pagine: 677 - 684
SICI:
0950-5423(200108)36:6<677:IOE(EL>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
APPLE POLYPHENOLOXIDASE; OXIDASE; PURIFICATION; TROPOLONE; JUICE;
Keywords:
enzymatic browning; inhibition; papaya latex; polyphenoloxidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Richard-Forget, F INRA, UBTAR, BP 81, F-33883 Villenave Dornon, France INRA BP 81 Villenave Dornon France F-33883 rnon, France
Citazione:
D. de Rigal et al., "Inhibition of endive (Cichorium endivia L.) polyphenoloxidase by a Carica papaya latex preparation", INT J FOOD, 36(6), 2001, pp. 677-684

Abstract

When endive polyphenoloxidase (PPO) was incubated with a crude papaya latex extract, it rapidly lost its activity. Inactivation was ascribed to thermostable nonenzymatic factors of low molecular weight. These factors were partially purified by a two step protocol including gel filtration chromatography on Biogel P2 and ion exchange chromatography using DEAE Sephadex A25. The PPO-inactivation rate was first order, when either inactivating agent or proton concentration was evaluated. Inactivation could be partially reversed by CuSO4, which suggested that the inactivating factor(s) bound to the copper site of the enzyme. On a more rapid time scale than inactivation, papaya latex extract acted also as a weak noncompetitive PPO inhibitor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:01:43