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Titolo:
Characterization of the human Fc gamma RIIB gene promoter: human zinc-finger proteins (ZNF140 and ZNF91) that bind to different regions function as transcription repressors
Autore:
Nishimura, T; Narita, T; Miyazaki, E; Ito, T; Nishimoto, N; Yoshizaki, K; Martial, JA; Bellfroid, EJ; Vissing, H; Taniyama, T;
Indirizzi:
Natl Inst Infect Dis, Dept Immunol, Immunoregulat Lab, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 gulat Lab, Tokyo 1628640, Japan Osaka Univ, Dept Immunol, Sch Hlth & Sport Sci, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ort Sci, Suita, Osaka 5650871, Japan Univ Liege, Inst Chem B6, Lab Mol Biol & Genet, B-4000 Sart Tilman Par Liege, Belgium Univ Liege Sart Tilman Par Liege Belgium B-4000 ilman Par Liege, Belgium Novo Nordisk AS, Mol & Cellular Biol, DK-2880 Bagsvaerd, Denmark Novo Nordisk AS Bagsvaerd Denmark DK-2880 ol, DK-2880 Bagsvaerd, Denmark
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 8, volume: 13, anno: 2001,
pagine: 1075 - 1084
SICI:
0953-8178(200108)13:8<1075:COTHFG>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR GENE; RESPONSE ELEMENTS; CELL-LINE; EXPRESSION; IGG; INTERFERON; KRUPPEL; ORGANIZATION; MACROPHAGES; ACTIVATION;
Keywords:
Fc gamma receptor II; repressor; transcription; zinc-finger protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Taniyama, T Natl Inst Infect Dis, Dept Immunol, Immunoregulat Lab, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 okyo 1628640, Japan
Citazione:
T. Nishimura et al., "Characterization of the human Fc gamma RIIB gene promoter: human zinc-finger proteins (ZNF140 and ZNF91) that bind to different regions function as transcription repressors", INT IMMUNOL, 13(8), 2001, pp. 1075-1084

Abstract

Expression of the human low-affinity Fc receptors for IgG (human Fc gamma RII) is differentially regulated. We report here the characterization of the promoter structure of the human Fc gamma RIIB gene and the isolation of the promoter region-binding proteins by a yeast one-hybrid assay. The minimal 154-bp region upstream from the transcription start site of the human Fc gamma RIIB gene was shown to possess promoter activity in a variety of cells. An electrophoretic mobility shift assay indicated that multiple nuclear factors in cell extracts bind to the two regions [F2-3 (-110 to -93) and F4-3 (-47 to -31)] of the human Fc gamma RIIB gene promoter. Mutation analysis indicated that GGGAGGAGC (-105 to -97) and AATTTGTTTGCC (-47 to -36) sequences are responsible for binding to nuclear factors respectively. By usingGGGAGGAGC and AATTTGTTTGCC as bait sequences, we cloned two zinc-finger proteins (ZNF140 and ZNF91) that bind to the F2-3 and F4-3 regions within thepromoter of the human Fc gamma RIIB gene respectively. When the ZNF140 andZNF91 were transfected with reporter plasmid, both showed repressor activity with additive effects. Thus, these results indicate that these cloned ZNF140 and ZNF91 proteins function as repressors for the human Fc gamma RIIB transcription.

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Documento generato il 04/07/20 alle ore 21:27:02