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Titolo:
IL-1 beta induces dendritic cells to produce IL-12
Autore:
Wesa, AK; Galy, A;
Indirizzi:
Wayne State Univ, Barbara Ann Karmanos Canc Inst, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 os Canc Inst, Detroit, MI 48201 USA
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 8, volume: 13, anno: 2001,
pagine: 1053 - 1061
SICI:
0953-8178(200108)13:8<1053:IBIDCT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CD34(+) PROGENITOR CELLS; C-KIT LIGAND; ENDOTHELIAL-CELLS; INTERFERON-GAMMA; IFN-GAMMA; T-CELLS; KAPPA-B; INTERLEUKIN-12; RECEPTOR; EXPRESSION;
Keywords:
CD40 ligand; cytokines; dendritic cells; human; IL-1; IL-12; IL-10; lipopolysaccharide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Galy, A Wayne State Univ, Barbara Ann Karmanos Canc Inst, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 Inst, Detroit, MI 48201 USA
Citazione:
A.K. Wesa e A. Galy, "IL-1 beta induces dendritic cells to produce IL-12", INT IMMUNOL, 13(8), 2001, pp. 1053-1061

Abstract

The cytokine IL-12, a product of dendritic cells (DC), plays a major role in cellular immunity, notably by inducing lymphocytes to produce IFN-gamma. Microbial products, T cell signals and cytokines induce the production of IL-12. Here, IL-1 beta is identified as a new IL-12-inducing agent, acting conjointly with CD40 ligand (CD40L) on human monocyte-derived DC in vitro. The effects of IL-1 beta were dose dependent, specifically blocked by neutralizing antibodies, and were observed both in immature and mature DC. Immature DC secreted more IL-12 than mature DC, but the effects of IL-1 beta were not due to a block of DC maturation as determined by analysis of DC surface markers. The mechanisms of action of IL-1 beta could be contrasted to that of other inducers of IL-12 such as IFN-gamma and lipopolysaccharide (LPS). Either IL-1 beta or IFN-gamma co-induced IL-12 with CD40L but conjointly, IL-1 beta, CD40L and IFN-gamma synergized, inducing very high levels of IL-12. The effects of IL-1 beta differed from those of LPS in that IL-1 beta, unlike LPS, could not induce IL-12 solely after IFN-gamma priming; and when combined with CD40L, IL-1 beta, unlike LPS, induced little IL-10. The mechanism of action of IL-1 beta involves IL-12 alpha mRNA up-regulation, andwe show that the combination of CD40L and IL-10 induces high levels of IL-12 alpha and IL-12 beta mRNA in DC. Altogether, these results delineate a new mechanism linking adaptive and innate immune responses for the regulation of IL-12 production in DC and for the role of IL-1 beta in the development of cellular immunity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:34:20