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Titolo:
Typing of Aeromonas isolates from children with diarrhoea & water samples by randomly amplified polymorphic DNA polymerase chain reaction & whole cell protein fingerprinting
Autore:
Alavandi, SV; Ananthan, S; Pramod, NP;
Indirizzi:
Univ Madras, Dr ALM Postgrad Inst Basic Med Sci, Dept Microbiol, Chennai 600113, India Univ Madras Chennai India 600113 , Dept Microbiol, Chennai 600113, India
Titolo Testata:
INDIAN JOURNAL OF MEDICAL RESEARCH
, volume: 113, anno: 2001,
pagine: 85 - 97
SICI:
0971-5916(200103)113:<85:TOAIFC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLONAL IDENTIFICATION; HYBRIDIZATION GROUPS; GEL-ELECTROPHORESIS; ARBITRARY PRIMERS; HYDROPHILA; STRAINS; RAPD; GASTROENTERITIS; PATTERNS; PCR;
Keywords:
Aeromonas; cluster analysis; dendrogram; diarrhoea; domestic water; epidemiology; protein fingerprinting; RAPD-PCR; SDS-PAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Ananthan, S Univ Madras, Dr ALM Postgrad Inst Basic Med Sci, Dept Microbiol, Taramani Campus, Chennai 600113, India Univ Madras Taramani Campus Chennai India 600113 00113, India
Citazione:
S.V. Alavandi et al., "Typing of Aeromonas isolates from children with diarrhoea & water samples by randomly amplified polymorphic DNA polymerase chain reaction & whole cell protein fingerprinting", I J MED RES, 113, 2001, pp. 85-97

Abstract

Background & objectives : Aeromonas spp. are water-borne organisms, often associated with childhood diarrhoea. The present study was conducted to examine the epidemiological relationship among the Aeromonas spp. isolated from water and children with acute diarrhoea in Chennai. Methods : Thirty six Aeromonas isolates inclusive of 16 from children withdiarrhoea, 15 from domestic water samples and 5 reference strains were studied by randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). Twenty eight Aeromonas isolates, 15 from children with diarrhoea, 10 from domestic water samples and three reference strains were analysed by SDS-PAGE for their whole cell protein profiles. Results : The 36 Aeromonas isolates examined by RAPD-PCR generated RAPD fingerprints with majority of the bands ranging from about 250 to 2800 bp. The RAPD fingerprints did not correspond with the phenospecies and varied greatly among the strains within the phenospecies. Cluster analysis revealed two major groups at 75 per cent hierarchical level, comprising 18 Aeromonas isolates, mainly recovered from domestic water samples, while the clinical isolates were scattered in different hierarchical levels in the dendrogram. The whole cell protein fingerprints examined by SDS-PAGE (lid not correspond with the phenospecies. Only four isolates of A. caviae were found to produce similar protein fingerprints allowing them to form a cluster at about 90 per cent hierarchical level, while the rest of the isolates were scattered it various hierarchical levels in the dendrogram. Interpretation & conclusions : In the present study, RAPD fingerprinting was found to be useful in distinguishing Aeromonas isolates recovered from clinical and domestic water supplies. However, RAPD-PCR could not distinguish the phenospecies of the genus Aeromonas. Whole cell protein fingerprinting and cluster analysis could neither differentiate isolates from clinical and domestic water sources nor the phenospecies of the genus Aeromonas.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 10:07:30