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Titolo:
Establishment of a human hepatocyte line (OUMS-29) having CYP 1A1 and 1A2 activities from fetal liver tissue by transfection of SV-10 LT
Autore:
Fukaya, KI; Asahi, S; Nagamori, S; Sakaguchi, M; Gao, C; Miyazaki, M; Namba, M;
Indirizzi:
Okayama Univ, Sch Med, Inst Mol & Cellular Biol, Dept Cell Biol, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 ept Cell Biol, Okayama 7008558, Japan Takeda Chem Ind Ltd, Osaka 5408645, Japan Takeda Chem Ind Ltd Osaka Japan 5408645 em Ind Ltd, Osaka 5408645, Japan Jikei Univ, Sch Med, Dept Internal Med 1, Tokyo 1058461, Japan Jikei UnivTokyo Japan 1058461 Dept Internal Med 1, Tokyo 1058461, Japan
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 5, volume: 37, anno: 2001,
pagine: 266 - 269
SICI:
1071-2690(200105)37:5<266:EOAHHL>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEM-CELLS; SIMIAN VIRUS-40; T-ANTIGEN; ALBUMIN GENE; BONE-MARROW; SYSTEM; IMMORTALIZATION; EXPRESSION; CULTURE; MICE;
Keywords:
human liver cell line; CYP 1A1; aromatic hydrocarbon receptor; aromatic hydrocarbon receptor nuclear translocator; carcinogenic carcinogens; 7-ethoxyresorufin deethylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Namba, M Okayama Univ, Sch Med, Inst Mol & Cellular Biol, Dept Cell Biol, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 Biol, Okayama 7008558, Japan
Citazione:
K.I. Fukaya et al., "Establishment of a human hepatocyte line (OUMS-29) having CYP 1A1 and 1A2 activities from fetal liver tissue by transfection of SV-10 LT", IN VITRO-AN, 37(5), 2001, pp. 266-269

Abstract

Immortalized human hepatocytes that can retain functions of drug-metabolizing enzymes would be useful for medical and pharmacological studies and forconstructing an artificial liver. The aim of this study wa. to establish immortalized human hepatocyte lines having differentiated liver-specific functions. pSVneo deoxyribonucleic acid, which contains large and small T genes in the early region of simian virus 40, was introduced into hepatocytes that had been obtained from the liver of a 21-wk-old fetus. Neomycin-resistant immortalized colonies were cloned and expanded to mass cultures to examine hepatic functions. Cells were cultured in a chemically defined serum-free medium, ASF101, which contains no peptides other than recombinant human transferrin and insulin. As a result, air immortal human hepatocyte cell line (OUMS-29) having li er-specific functions was established from one of the13 clones. Expression of CYP 1A1 arid 1A2 messenger ribonucleic acid by the cells was induced by treatment with benz[a]pyrene, 3-methylcholanthrene, and benz[a]anthracene. OUMS-29 cells had both the polycyclic aromatic hydrocarbon receptor (AhR) arid AhR nuclear translocator. Consequently, 7-ethoxyresorufin deethylase activity of the cells was induced time- and dose-dependent by these polycyclic aromatic hydrocarbons. This cell line is expected to he instrumental as an alternative method in animal experiments for studying hepatocarcinogenesis. drug metabolisms of liver cells, and hepatic toxicology,.

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Documento generato il 29/03/20 alle ore 08:56:21