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Titolo:
Hemolymph analysis and evaluation of newly formulated media for culture ofshrimp cells (Penaeus stylirostris)
Autore:
Shimizu, C; Shike, H; Klimpel, KR; Burns, JC;
Indirizzi:
Univ Calif San Diego, Sch Med, Dept Pediat 0830, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 t 0830, La Jolla, CA 92093 USA Super Shrimp Inc, National City, CA 91950 USA Super Shrimp Inc National City CA USA 91950 , National City, CA 91950 USA
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 6, volume: 37, anno: 2001,
pagine: 322 - 329
SICI:
1071-2690(200106)37:6<322:HAAEON>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANION-EXCHANGE CHROMATOGRAPHY; PULSED AMPEROMETRIC DETECTION; MONOSACCHARIDE ANALYSIS; PRAWN; PROLIFERATION; VANNAMEI; TISSUES; MONODON;
Keywords:
crustacean cell culture; hemolymph analysis; protein synthesis; DNA synthesis; Penaeus stylirostris;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Burns, JC Univ Calif San Diego, Sch Med, Dept Pediat 0830, 9500 Gilman Dr,La Jolla,CA 92093 USA Univ Calif San Diego 9500 Gilman Dr La Jolla CA USA 92093 93 USA
Citazione:
C. Shimizu et al., "Hemolymph analysis and evaluation of newly formulated media for culture ofshrimp cells (Penaeus stylirostris)", IN VITRO-AN, 37(6), 2001, pp. 322-329

Abstract

Creation of a shrimp cell line has been an elusive goal. This failure may be due to the composition of the cell culture medium, which may be inadequate to support primary cultured cells. Shrimp hemolymph should contain the nutritional components needed to support cell growth and division. We reporthere the comprehensive biochemical analysis of hemolymph from the blue shrimp, Penaeus stylirostris (Litopenaeus stylirostris) (see Holthuis, L. B. Shrimps and prawns of the world, in: FAO species catalog. Vol. 1. Rome: Foodand Agriculture Organization of the United Nations; 1980), for free amino acids (FAAs), carbohydrates, electrolytes, metals, pH, and osmolality. Levels of hemolymph components were compared to 2XL-15 with 20% fetal bovine serum, a commonly used culture medium for crustacean cells. The FAAs, taurineand proline, and the metals, strontium and zinc, were significantly higherin hemolymph than in the 2XL-15 medium. In contrast, other FAAs were up to50 times higher in the 2XL-15 medium than in the hemolymph. To mimic more closely the hemolymph composition, we created two new media based on eitherthe 0.2XL-15 or the M199 medium. We compared the microscopic appearance ofcells cultured in these media and evaluated deoxyribonucleic acid (DNA) and protein synthesis by H-3-thymidine uptake and S-35-methionine uptake assays. The ovary cells of P. stylirostris cultured in either of the new media formed monolayers, while the cells cultured in 2XL-15 medium did not. Despite these differences, there was no evidence of sustained DNA or protein synthesis with any of the media. Future studies to establish a shrimp cell line should focus on analysis of the cell cycle and on overcoming the molecular blocks to cell division.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:10:01