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Titolo:
In vivo electroporation-mediated transfer of interleukin-12 and interleukin-18 genes induces significant antitumor effects against melanoma in mice
Autore:
Kishida, T; Asada, H; Satoh, E; Tanaka, S; Shinya, M; Hirai, H; Iwai, M; Tahara, H; Imanishi, J; Mazda, O;
Indirizzi:
Kyoto Prefectural Univ Med, Dept Microbiol, Kamikyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med, Dept Internal Med 3, Kamikyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 Ku, Kyoto 6028566, Japan Univ Tokyo, Inst Med Sci, Dept Surg, Tokyo, Japan Univ Tokyo Tokyo Japan niv Tokyo, Inst Med Sci, Dept Surg, Tokyo, Japan
Titolo Testata:
GENE THERAPY
fascicolo: 16, volume: 8, anno: 2001,
pagine: 1234 - 1240
SICI:
0969-7128(200108)8:16<1234:IVETOI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPSTEIN-BARR-VIRUS; RECOMBINANT HUMAN INTERLEUKIN-12; IN-VIVO; POLYAMIDOAMINE DENDRIMER; NUCLEAR ANTIGEN-1; PANCREATIC-CANCER; GAMMA PRODUCTION; CARCINOMA CELLS; ELECTRIC PULSE; PHASE-I;
Keywords:
electroporation; gene therapy; cytokine; Epstein-Barr virus-based vector; episomal vector; melanoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Mazda, O Kyoto Prefectural Univ Med, Dept Microbiol, Kamikyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 o 6028566, Japan
Citazione:
T. Kishida et al., "In vivo electroporation-mediated transfer of interleukin-12 and interleukin-18 genes induces significant antitumor effects against melanoma in mice", GENE THER, 8(16), 2001, pp. 1234-1240

Abstract

Direct intratumoral transfection of cytokine genes was performed by means of the in vivo electroporation as a novel therapeutic strategy for cancer. Plasmid vectors carrying the firefly luciferase, interleukin (IL)-12 and IL-18 genes were injected into established subcutaneous B16-derived melanomasfollowed by electric pulsation. When plasmid vectors with Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) gene were employed, the expression levels of the transgenes were significantly higher in comparison with those obtained with conventional plasmid vectors. In consequence of the transfection with IL-12 and IL-18 genes, serum concentrations of the cytokines were significantly elevated, while interferon (IFN)-gamma also increased in the seraof the animals. The IL-12 gene transfection resulted in significant suppression of tumor growth, while the therapeutic effect was further improved byco-transfection with IL-12 and IL-18 genes. Repetitive co-transfection with IL-12 and IL-18 genes resulted in significant prolongation of survival ofthe animals. Natural killer (NK) and cytotoxic T lymphocyte (CTL) activities were markedly enhanced in the mice transfected with the cytokine genes. The present data suggest that the cytokine gene transfer can be successfully achieved by in vivo electroporation, leading to both specific and nonspecific antitumoral immune responses and significant therapeutic outcome.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:01:48