Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Release of soluble tryptase but only minor amounts of chymase activity from cutaneous mast cells
Autore:
Kivinen, PK; Kaminska, R; Naukkarinen, A; Harvima, RJ; Horsmanheimo, M; Harvima, IT;
Indirizzi:
Kuopio Univ Hosp, Dept Dermatol, Kuopio 70211, Finland Kuopio Univ Hosp Kuopio Finland 70211 pt Dermatol, Kuopio 70211, Finland Kuopio Univ Hosp, Dept Pathol, SF-70210 Kuopio, Finland Kuopio Univ Hosp Kuopio Finland SF-70210 athol, SF-70210 Kuopio, Finland
Titolo Testata:
EXPERIMENTAL DERMATOLOGY
fascicolo: 4, volume: 10, anno: 2001,
pagine: 246 - 255
SICI:
0906-6705(200108)10:4<246:ROSTBO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-SKIN; PROTEASE INHIBITORS; CLEAVAGE; PROTEINASES; ACTIVATION; HISTAMINE; PROCOLLAGENASE; IDENTIFICATION; DEGRANULATION; FIBRONECTIN;
Keywords:
mediator release; protease; protease inhibitor; skin blister; skin culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Harvima, IT Kuopio Univ Hosp, Dept Dermatol, POB 1777, Kuopio 70211, Finland Kuopio Univ Hosp POB 1777 Kuopio Finland 70211 70211, Finland
Citazione:
P.K. Kivinen et al., "Release of soluble tryptase but only minor amounts of chymase activity from cutaneous mast cells", EXP DERMATO, 10(4), 2001, pp. 246-255

Abstract

Tryptase and chymase are the major serine proteinases of skin mast cells but their biologic significance depends on their activity. In this study, wedemonstrate the release of soluble activity of tryptase, but not markedly that of chymase, into skin blister fluids induced by freezing with liquid nitrogen as well as into supernatant during incubation of 8 whole skin specimens with compound 48/80 for up to 2 days followed by sonication. Incubation of 3 other skin specimens in compound 48/80 for up to 2 days revealed that the number of mast cells displaying tryptase activity decreased significantly on day 2, and the number of mast cells showing chymase activity (but not those showing chymase immunoreactivity) decreased significantly on day Ibut not thereafter on day 2. The results of 3 skin organ cultures for up to 14 days showed steady decrease in the number of tryptase-positive cells but persistence of mast cells containing chymase activity. Chymase in solution was sensitively inhibited by 0.01 mg/ml alpha (1)-antichymotrypsin but higher concentrations (0.3-3.0 mg/ml) were needed for inhibiting chymase on skin sections. In conclusion, after mast cell degranulation tryptase activity is substantially solubilized and it may potentially affect both local and distant skin structures. Instead, chymase is partially inactivated and the remaining chymase activity persists at the site of degranulation having only local effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:53:27