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Titolo:
LASA, ALKALINE PROTEASE AND ELASTASE IN CLINICAL STRAINS OF PSEUDOMONAS-AERUGINOSA - QUANTIFICATION BY IMMUNOCHEMICAL METHODS
Autore:
COIN D; LOUIS D; BERNILLON J; GUINAND M; WALLACH J;
Indirizzi:
UNIV LYON 1,LAB BIOCHIM ANALYT & SYNTHESE BIOORGAN,43 BD 11 NOVEMBRE 1918 F-69622 VILLEURBANNE FRANCE
Titolo Testata:
FEMS immunology and medical microbiology
fascicolo: 3, volume: 18, anno: 1997,
pagine: 175 - 184
SICI:
0928-8244(1997)18:3<175:LAPAEI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXOTOXIN-A; NUCLEOTIDE-SEQUENCE; NEUTRALIZING ANTIBODIES; GENE; EXPRESSION; PURIFICATION; INACTIVATION; CLEAVAGE; ALGINATE; CLONING;
Keywords:
PSEUDOMONAS AERUGINOSA; MULTIPLE ANTIGEN PEPTIDE; LASA; ALKALINE PROTEASE; ELASTASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
D. Coin et al., "LASA, ALKALINE PROTEASE AND ELASTASE IN CLINICAL STRAINS OF PSEUDOMONAS-AERUGINOSA - QUANTIFICATION BY IMMUNOCHEMICAL METHODS", FEMS immunology and medical microbiology, 18(3), 1997, pp. 175-184

Abstract

Thirty Pseudomonas aeruginosa strains were isolated from the sputa ofcystic fibrosis patients. In each culture supernatant, the amount of three exoproteases (LasA, alkaline protease and elastase) was determined using immunochemical procedures. These assays used selected peptide-MAP (multiple antigen peptide) strategy as antigen for animal immunisation. The method appeared to be reproducible, simple, sensitive and specific without cross-reactivity between the antisera. The resulting values differed from one strain to another mostly for elastase production. Despite the fact that four genes (lasA, lasB, lasR and rhlR) were shown to be necessary for full elastolytic activity, it was obvious that if LasA was not secreted in a naturally non-elastase-producing strain, in return in an elastase-producing strain, there were no apparent relationships between LasA and elastase production and between LasA and alkaline protease secretion. Furthermore, in vitro, the secretion ofthe three exoproteases seemed to be independent of the mucoid or non-mucoid phenotype of the bacteria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 19:55:39