Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
D-2-like dopamine receptor activation excites rat dorsal raphe 5-HT neurons in vitro
Autore:
Haj-Dahmane, S;
Indirizzi:
SUNY Buffalo, Res Inst Addict, Buffalo, NY 14203 USA SUNY Buffalo BuffaloNY USA 14203 Res Inst Addict, Buffalo, NY 14203 USA
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 1, volume: 14, anno: 2001,
pagine: 125 - 134
SICI:
0953-816X(200107)14:1<125:DDRAER>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEUS ACCUMBENS NEURONS; VENTRAL TEGMENTAL AREA; IN-VIVO MICRODIALYSIS; CA1 PYRAMIDAL CELLS; FREELY MOVING RATS; POTASSIUM CONDUCTANCE; SUBSTANTIA NIGRA; D-2 RECEPTORS; HORSERADISH-PEROXIDASE; SEROTONERGIC NEURONS;
Keywords:
membrane depolarization; nonselective cation current; whole-cell recording;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Haj-Dahmane, S SUNY Buffalo, Res Inst Addict, 1021 Main St, Buffalo, NY 14203 USA SUNY Buffalo 1021 Main St Buffalo NY USA 14203 NY 14203 USA
Citazione:
S. Haj-Dahmane, "D-2-like dopamine receptor activation excites rat dorsal raphe 5-HT neurons in vitro", EUR J NEURO, 14(1), 2001, pp. 125-134

Abstract

The aim of the present study was to investigate the effect of dopamine (DA) on the excitability of dorsal raphe nucleus (DRN) 5-hydroxytryptamine (5-HT) neurons using the patch-clamp technique in brain slices. Bath application of DA (1-300 mum) produced a concentration-dependent membrane depolarization in all 5-HT neurons examined. This effect persisted in the presence oftetrodotoxin (TTX; 1 mum) and low extracellular calcium. Moreover, blockade of ionotropic glutamate receptors with 6,7-dinitroquinoxaline-2,3-dione (DNQX) and 2-amino-5-phosphonopentanoic acid (AP5) did not prevent DA-induced depolarization, indicating that it was mediated by a direct effect of DA on 5-HT neurons. The DA-induced depolarization was not antagonized by selective alpha (1)-adrenergic receptor antagonists, prazosin and WB 4101, but by a nonselective DA receptor antagonist, haloperidol. In addition, the selective D-2-like receptor agonist quinpirole and antagonist sulpiride mimicked and blocked DA-induced depolarization, respectively. These results indicate that DA-induced membrane depolarization in DRN 5-HT neurons is mediated by the activation of D-2-like DA receptors. The DA-induced membrane depolarization and inward current were associated with an increase in membrane conductance. Examination of the current-voltage (I-V) relationship for the DA-induced inward current revealed that the amplitude of the current increasedwith membrane hyperpolarization and reversed polarity at a potential near -15 mV. These data suggest that DA-induced depolarization in DRN 5-HT neurons is not mediated by a decrease in potassium conductance, but most likely by the activation of a nonselective cation current.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:01:45