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Titolo:
Expression and regulation of leptin receptor proteins in afferent and efferent neurons of the vagus nerve
Autore:
Buyse, M; Ovesjo, ML; Goiot, H; Guilmeau, S; Peranzi, G; Moizo, L; Walker, F; Lewin, MJM; Meister, B; Bado, A;
Indirizzi:
Univ Paris 07, INSERM, U410, Paris, France Univ Paris 07 Paris FranceUniv Paris 07, INSERM, U410, Paris, France Karolinska Inst, Dept Neurosci, Stockholm, Sweden Karolinska Inst Stockholm Sweden Inst, Dept Neurosci, Stockholm, Sweden Hop Bichat Claude Bernard, Serv Cytol & Anat Pathol, F-75877 Paris 18, France Hop Bichat Claude Bernard Paris France 18 thol, F-75877 Paris 18, France
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 1, volume: 14, anno: 2001,
pagine: 64 - 72
SICI:
0953-816X(200107)14:1<64:EAROLR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOOD-INTAKE; BODY-WEIGHT; OBESE GENE; ENDOGENOUS CHOLECYSTOKININ; SYNERGISTIC INTERACTION; ARCUATE NUCLEUS; BINDING-SITES; DIABETIC MICE; MESSENGER-RNA; HUMAN STOMACH;
Keywords:
DMNX; nodose ganglion; NTS; Ob-Rb; STAT3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Bado, A Univ Paris 07, INSERM, U410, Paris, France Univ Paris 07 Paris France aris 07, INSERM, U410, Paris, France
Citazione:
M. Buyse et al., "Expression and regulation of leptin receptor proteins in afferent and efferent neurons of the vagus nerve", EUR J NEURO, 14(1), 2001, pp. 64-72

Abstract

Leptin, the product of the ob gene, plays a key role in the regulation of food intake via a cross-talk between hypothalamic leptin receptors and neuropeptides that affect feeding behaviour. Recent studies have shown a synergistic interaction between leptin and cholecystokinin (CCK) leading to suppression of food intake, which involves CCK-1 receptors and capsaicin-sensitive vagal fibres. In this study, we have investigated the presence of leptinreceptors in afferent and efferent neurons of the vagus nerve. By using reverse transcription-polymerase chain reaction, mRNAs encoding long (Ob-Rb) and short (Ob-Ra) leptin receptor isoforms were detected in the rat nodose ganglion, which contains the cell bodies of the vagal afferent neurons. Western blot analysis confirmed the presence of leptin receptor-immunoreactiveproteins in extracts from the vagal trunk. Immunohistochemistry showed thepresence of leptin receptors and the leptin-induced transcription factor STAT3 in the cytoplasm of nodose ganglion cells. In cervical vagal segments,levels of leptin receptor protein displayed physiological regulation, withdecreased amounts after feeding and increased levels after food restriction. In addition, leptin receptor and STAT3 immunoreactivities were detected in neurons of the nucleus of tractus solitarius (NTS) and the dorsal motor nucleus of the vagus nerve (DMNX) by immunofluorescence histochemistry. Furthermore, direct double-labelling demonstrated colocalization of Ob-Rb and STAT3 immunoreactivities in cholinergic vagal efferent cell bodies of the DMNX. It is speculated that vagal leptin receptors, apart from being activated by adipocyte-derived leptin, may also be influenced by leptin produced by the stomach. This may explain the synergistic action of leptin and CCK onneuronal activity in the NTS and on food intake.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:06:38