Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Isolation, purification and partial characterization of early pregnancy factor (EPF) from sera of pregnant women
Autore:
Haq, A; Mothi, BA; Al-Hussein, K; Al-Tufail, M; Hollanders, J; Jaroudi, K; Al-Waili, N; Shabani, M;
Indirizzi:
Dubai Specialized Med Ctr & Res Labs, Dubai, U Arab Emirates Dubai Specialized Med Ctr & Res Labs Dubai U Arab Emirates rab Emirates King Faisal Specialist Hosp & Res Ctr, Riyadh 11211, Saudi Arabia King Faisal Specialist Hosp & Res Ctr Riyadh Saudi Arabia 11211 i Arabia
Titolo Testata:
EUROPEAN JOURNAL OF MEDICAL RESEARCH
fascicolo: 5, volume: 6, anno: 2001,
pagine: 209 - 214
SICI:
0949-2321(20010529)6:5<209:IPAPCO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CHORIONIC-GONADOTROPIN; ROSETTE INHIBITION TEST; FACTOR-LIKE MOLECULE; ACTIVE POLYPEPTIDES; EWES SERA; LYMPHOCYTES; SEARCH; MICE;
Keywords:
early pregnancy factor (EPF); rosette inhibition assay (RIA); sodium dodecyl sulphate; polyacrylamide gel electrophoresis (SDS-PAGE); ion exchange chromatography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Haq, A Dubai Specialized Med Ctr & Res Labs, POB 19099, Dubai, U Arab Emirates Dubai Specialized Med Ctr & Res Labs POB 19099 Dubai U Arab Emirates
Citazione:
A. Haq et al., "Isolation, purification and partial characterization of early pregnancy factor (EPF) from sera of pregnant women", EUR J MED R, 6(5), 2001, pp. 209-214

Abstract

Early pregnancy factor (EPF) is a pregnancy protein, which is secreted into the maternal serum 12-16 hours after fertilization. It is thought to be an immuno suppressive molecule. EPF is detected in pregnant woman's serum bythe rosette inhibition assay (RIA). In this study, EPF was purified from the pregnant woman's sera by using ion exchange chromatography and analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The proteins which showed a positive result with the RIA, were found to be 35 kDa and 17 kDa molecular weights. The biological activities of these proteins were stable upon heat treatment at 56 degreesC for 30 min. Proteins isolated and purified in this study might be of great significance to the field of human reproduction with particular reference to pregnancy and recurrent abortion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:11:30