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Titolo:
Chicken interleukin-6 - cDNA structure and biological properties
Autore:
Schneider, K; Klaas, R; Kaspers, B; Staeheli, P;
Indirizzi:
Univ Freiburg, Dept Virol, Inst Med Mikrobiol & Hyg, Abt Virol, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 Virol, D-79104 Freiburg, Germany Univ Munich, Inst Tierphysiol, D-80539 Munich, Germany Univ Munich Munich Germany D-80539 Tierphysiol, D-80539 Munich, Germany
Titolo Testata:
EUROPEAN JOURNAL OF BIOCHEMISTRY
fascicolo: 15, volume: 268, anno: 2001,
pagine: 4200 - 4206
SICI:
0014-2956(200108)268:15<4200:CI-CSA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; HUMAN-FIBROBLASTS; GROWTH-FACTOR; CELL-GROWTH; INTERFERON; DIFFERENTIATION; CYTOKINES; PROTEIN; FAMILY; EXPRESSION;
Keywords:
chicken; imidazoquinolines; suppression subtractive hybridization (SSH); interleukin-6;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Staeheli, P Univ Freiburg, Dept Virol, Inst Med Mikrobiol & Hyg, Abt Virol, Hermann Herder Str 11, D-79104 Freiburg, Germany Univ Freiburg Hermann Herder Str 11 Freiburg Germany D-79104
Citazione:
K. Schneider et al., "Chicken interleukin-6 - cDNA structure and biological properties", EUR J BIOCH, 268(15), 2001, pp. 4200-4206

Abstract

Suppression subtractive hybridization technology was used to identify differentially expressed genes in spleens of chickens that had been treated with the synthetic immune modifier S-28463. One induced chicken gene encoded aprotein with about 35% sequence identity to human interleukin-6 (IL-6). Itconsists of 241 amino acids including a putative N-terminal signal peptideof 47 residues. Bacterially expressed chicken IL-6 (ChIL-6) carrying a histidine tag in place of the signal peptide was biologically active: it induced proliferation of the IL-6-dependent murine hybridoma cell line 7TD1. Theconcentration of ChIL-6 required for half-maximal proliferative response was approximate to 60 pg.mL(-1). When injected intravenously into adult chickens, purified recombinant ChIL-6 induced an increase in serum corticosterone levels. Supernatants of chicken LMH and monkey COS-7 cells transiently transfected with a ChIL-6 expression construct induced proliferation of 7TD1cells, demonstrating that recombinant ChIL-6 from eukaryotic cells is alsoactive.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 20:56:47