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Titolo:
The novel untranslated exon "exon 0T" encoded between the exon 0 and exon 1 of the rat estrogen receptor alpha (ER alpha) gene
Autore:
Osada, N; Hirata, S; Shoda, T; Hoshi, K;
Indirizzi:
Yamanashi Univ, Dept Obstet & Gynecol, Yamanashi 4093898, Japan Yamanashi Univ Yamanashi Japan 4093898 Gynecol, Yamanashi 4093898, Japan
Titolo Testata:
ENDOCRINE JOURNAL
fascicolo: 4, volume: 48, anno: 2001,
pagine: 465 - 472
SICI:
0918-8959(200108)48:4<465:TNUE"0>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CDNA; IDENTIFICATION; TRANSCRIPTS; EXPRESSION;
Keywords:
estrogen receptor alpha gene; messenger ribonucleic acid; untranslated exon; alternative splicing; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Hoshi, K Yamanashi Univ, Dept Obstet & Gynecol, Shimokato 1110, Yamanashi 4093898, Japan Yamanashi Univ Shimokato 1110 Yamanashi Japan 4093898 898, Japan
Citazione:
N. Osada et al., "The novel untranslated exon "exon 0T" encoded between the exon 0 and exon 1 of the rat estrogen receptor alpha (ER alpha) gene", ENDOCR J, 48(4), 2001, pp. 465-472

Abstract

We have recently isolated two untranslated first exons, exon 0N and exon 0S, of rat estrogen receptor alpha (ER alpha) gene from the liver by use of 5 ' -rapid amplification of the cDNA ends (5 ' -RACE) method. In this communication, we further analyzed the 5 ' -untranslated region (UTR) of the ER alpha mRNA in rat anterior hypophysis in order to investigate the existenceof the other 5 ' -untranslated exon(s) of rat ER alpha gene. Total RNA from the anterior hypophysis of 8-week-old female Wistar strain male rats was subjected to 5 ' -RACE with antisense primers located in exon 1 of the rat ER alpha gene and one of the positive clones (clone 35) was sequenced. The nucleotide sequence of clone 35 revealed the insertion of a previously unidentified exon (which we termed "exon 0T") between exon 0 (the first reported 5 ' -UTR form of rat ER alpha mRNA) and exon 1 of rat ER alpha mRNA. Analysis of rat genomic DNA indicated that exon 0T was located between exon 0 and exon I of rat ER alpha gene. We further investigated the distribution ofER alpha mRNA containing exon 0T in several brain regions and various peripheral tissues of 8-week-old male and female Wistar strain rats by use of reverse transcription-polymerase chain reaction (RT-PCR). The distribution of the ER alpha mRNA (0T-1) was essentially similar to that of ER alpha mRNAin which exon 0 was spliced onto exon 1 reported previously. These resultsindicate that (1) exon 0T is a novel untranslated exon of rat ERa gene which is located between exon 0 and exon I on rat genomic DNA, (2) exon 0T is inserted between exon 0 and exon 1 of ER alpha mRNA by alternative splicing, and (3) this alternative splicing may occur in tissues where the transcription of ER alpha gene is initiated from exon 0.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:14:54