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Titolo:
The perplexed and confused mutations affect distinct stages during the transition from proliferating to post-mitotic cells within the zebrafish retina
Autore:
Link, BA; Kainz, PM; Ryou, T; Dowling, JE;
Indirizzi:
Harvard Univ, Dept Mol & Cell Biol, Cambridge, MA 02138 USA Harvard Univ Cambridge MA USA 02138 & Cell Biol, Cambridge, MA 02138 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 236, anno: 2001,
pagine: 436 - 453
SICI:
0012-1606(20010815)236:2<436:TPACMA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DANIO-RERIO; IN-VIVO; FATE DETERMINATION; VERTEBRATE RETINA; EMBRYONIC RETINA; PROGENITOR CELLS; EYE DEVELOPMENT; HOMEOBOX GENES; AMACRINE CELLS; CHICK RETINA;
Keywords:
retina; lamination; differentiation; apoptosis; neuroepithelium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Link, BA Harvard Univ, Dept Mol & Cell Biol, Cambridge, MA 02138 USA Harvard Univ Cambridge MA USA 02138 iol, Cambridge, MA 02138 USA
Citazione:
B.A. Link et al., "The perplexed and confused mutations affect distinct stages during the transition from proliferating to post-mitotic cells within the zebrafish retina", DEVELOP BIO, 236(2), 2001, pp. 436-453

Abstract

To identify and study genes essential for vertebrate retinal development, we are screening zebrafish embryos for mutations that disrupt retinal histogenesis. Key steps in retinogenesis include withdrawal from mitosis by multipotent neuroepithelial cells, specification to particular cell types, migration to the appropriate laminar positions, and molecular and morphologicaldifferentiation. In this study, we have identified two recessive mutationsthat affect the transition of proliferating neuroepithelial cells to postmitotic retinal cells. Both the perplexed and confused mutant phenotypes were initially detectable when the first retinal neuroepithelial cells began to leave the cell cycle. At this time, each mutant retina showed increased cell death and a lack of morphological differentiation. Cell death was foundto be apoptotic in both perplexed and confused retinas based on TUNEL analysis and activation of caspase-3. TUNEL-phosphoRb-BrdU colocalization studies indicated that the perplexed mutation caused death in cells transitioning from a proliferative to postmitotic state. For the confused mutation, TUNEL-phosphoRb-BrdU analysis revealed that only a subset of postmitotic cellswere induced to activate apoptosis. Mosaic analysis demonstrated that within the retina the perplexed mutation functions noncell-autonomously. Furthermore, whole lens or eye cup transplantations indicated that the retinal defect was intrinsic to the retina. Mosaic analysis with confused embryos showed this mutation acts cell-autonomously. From these studies, we conclude that the perplexed and confused genes are essential at distinct stages during the transition from proliferating to postmitotic cells within the zebrafish retina. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 19:27:39