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Titolo:
Characterization of a minisatellite repeat locus in the chloroplast genomeof Orchis palustris (Orchidaceae)
Autore:
Cafasso, D; Pellegrino, G; Musacchio, A; Widmer, A; Cozzolino, S;
Indirizzi:
Univ Naples Federico II, Dipartimento Biol Vegetale, I-80139 Naples, ItalyUniv Naples Federico II Naples Italy I-80139 tale, I-80139 Naples, Italy Univ Calabria, Dipartimento Ecol, I-87036 Cosenza, Italy Univ Calabria Cosenza Italy I-87036 timento Ecol, I-87036 Cosenza, Italy ETH Zurich, Geobot Inst, CH-8008 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8008 Inst, CH-8008 Zurich, Switzerland
Titolo Testata:
CURRENT GENETICS
fascicolo: 5-6, volume: 39, anno: 2001,
pagine: 394 - 398
SICI:
0172-8083(200107)39:5-6<394:COAMRL>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-I INTRON; VARIABLE NUMBER; DNA; PHYLOGENY; MUTATION; PRIMERS; ORIGIN; PLANTS; GENES;
Keywords:
chloroplast genome (cpDNA); minisatellite; Orchis palustris; variable number of tandem repeats (VNTR);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Cozzolino, S Univ Naples Federico II, Dipartimento Biol Vegetale, Via Foria 223, I-80139 Naples, Italy Univ Naples Federico II Via Foria 223 Naples Italy I-80139 y
Citazione:
D. Cafasso et al., "Characterization of a minisatellite repeat locus in the chloroplast genomeof Orchis palustris (Orchidaceae)", CURR GENET, 39(5-6), 2001, pp. 394-398

Abstract

We describe the occurrence of a tandem repeat in them chloroplast genome of the marsh orchid, Orchis palustris. The repeat unit is an AT-rich, 16-bp sequence located in the chloroplast tRNA(LEU) intron. Southern blot analysis confirmed that the O. palustris tRNA(LEU) intron including the minisatellite locus has not been transferred to the nucleus, but is indeed located onthe chloroplast genome. The 16-bp repeat unit was found to be present in all O. palustris accessions studied, as well as in the closely related O. laxiflora. Variation in repeat numbers among individuals was found in O. palustris from central and northern Italy; and this was consistently associatedwith a 13-bp sequence motif preceding the repeat. This motif was absent from O. palustris from southern Italy, Greece, and from O. laxiflora. In these accessions, no variation in repeat numbers was found. Our results suggestthat the O. palustris chloroplast minisatellite. locus evolved relatively recently, presumably in central Italy, and may represent a valuable marker for population genetic studies.

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Documento generato il 04/04/20 alle ore 09:02:57