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Titolo:
Melting temperature analysis as quantitative method for detection of pointmutations
Autore:
Porcelli, B; Frosi, B; Terzuoli, L; Arezzini, L; Pagani, R; Civitelli, S; Tanzini, G; Orlando, C; Pazzagli, M; Marziliano, N; Da Prato, L;
Indirizzi:
Univ Siena, Inst Biochem & Enzymol, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 t Biochem & Enzymol, I-53100 Siena, Italy Univ Siena, Inst Clin Surg, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 ena, Inst Clin Surg, I-53100 Siena, Italy Univ Florence, Dept Clin Physiopathol, Clin Biochem Unit, Florence, Italy Univ Florence Florence Italy pathol, Clin Biochem Unit, Florence, Italy Appl Biosyst Inc, SDS GA Core Lab, Monza, Italy Appl Biosyst Inc Monza Italy Biosyst Inc, SDS GA Core Lab, Monza, Italy
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 6, volume: 39, anno: 2001,
pagine: 501 - 504
SICI:
1434-6621(200106)39:6<501:MTAAQM>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; K-RAS MUTATIONS; CANCER; ONCOGENES; VARIANT;
Keywords:
melting temperature analysis; K-ras gene; colorectal cancer;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Porcelli, B Univ Siena, Inst Biochem & Enzymol, Via Aldo Moro, I-53100 Siena, Italy Univ Siena Via Aldo Moro Siena Italy I-53100 100 Siena, Italy
Citazione:
B. Porcelli et al., "Melting temperature analysis as quantitative method for detection of pointmutations", CLIN CH L M, 39(6), 2001, pp. 501-504

Abstract

Different methods have been devised to detect point mutations. Some are very sensitive, detecting mutations even in a background of normal tissue, but none provide information about the percentage of cells with mutant DNA. Here we describe an easy, fast and reliable method, melting temperature analysis, which not only detects point mutations but also provides quantitativeinformation on the percentage of cells with mutant DNA. By this method we detected a G-A transition in codon 12 of the K-ras gene in DNA of subjects with colorectal cancer. The K-ras mutation was found in 9/10 bowel cancers and 8/10 normal adjacent samples. It was also detected in 4/7 stool samplesfrom the same patients. In colorectal cancers, the proportion of K-ras mutant cells was variable: in two the mutant/wild-type DNA ratio was 30/70, inthree 50/50, and in four 70/30. Melting temperature analysis was sensitivefor the detection of point mutations in bowel cancer and also in apparently normal tissue, providing quantitative information about the percentage ofcells with mutant DNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 12:11:41