Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
An abnormal adherence of monocytes to fibronectin in thyroid autoimmunity has consequences for cell polarization and the development of veiled cells
Autore:
Canning, MO; Grotenhuis, K; De Haan-Meulman, M; De Wit, HJ; Berghout, A; Drexhage, HA;
Indirizzi:
Erasmus Univ, Dept Immunol, NL-3000 DR Rotterdam, Netherlands Erasmus Univ Rotterdam Netherlands NL-3000 DR DR Rotterdam, Netherlands Med Ctr Rijnmond Zuid, Dept Internal Med, Rotterdam, Netherlands Med Ctr Rijnmond Zuid Rotterdam Netherlands Med, Rotterdam, Netherlands
Titolo Testata:
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
fascicolo: 1, volume: 125, anno: 2001,
pagine: 10 - 18
SICI:
0009-9104(200107)125:1<10:AAAOMT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONOBESE DIABETIC MICE; COLONY-STIMULATING FACTOR; WISKOTT-ALDRICH-SYNDROME; NECROSIS-FACTOR-ALPHA; DENDRITIC CELLS; GRAVES-DISEASE; SIGNAL-TRANSDUCTION; BLOOD-LYMPHOCYTES; INTEGRINS; ANTIGEN;
Keywords:
monocytes; fibronectin; polarization; veiled cells; thyroid autoimmunity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Drexhage, HA Erasmus Univ, Dept Immunol, POB 1738, NL-3000 DR Rotterdam, Netherlands Erasmus Univ POB 1738 Rotterdam Netherlands NL-3000 DR lands
Citazione:
M.O. Canning et al., "An abnormal adherence of monocytes to fibronectin in thyroid autoimmunity has consequences for cell polarization and the development of veiled cells", CLIN EXP IM, 125(1), 2001, pp. 10-18

Abstract

Blood monocytes of patients with thyroid autoimmune disease (TAID) displaydefects in rearranging their cortical actomyosin cytoskeleton ('polarize')in response to chemoattractants. Such rearrangements also take place afterthe adherence of monocytes to the extracellular matrix (ECM). It is therefore not surprising that monocytes are primed after fibronectin (FN) adherence, displaying an enhanced polarization toward chemoattractants. We investigated the integrin expression and chemoattractant-induced polarization of monocytes of TAID patients before and after FN adherence. Since cytoskeletal rearrangements are also required during the transition of monocytes into veiled antigen-presenting cells (VCs), we investigated such transition of FN-adherent monocytes of TAID patients. Adherent and nonadherent monocyte populations from TAID patients and healthy controls were subjected to a polarization test with the chemoattractant fMLP (or MCP-1), FACS analyses (FITC-labelled FN, CD29, CD49e, d, b and a) and tested for their capability to develop into veiled APC. Monocytes of healthy individuals showed an improved chemoattractant-induced cell polarization after FN adherence, not reflected by TAID monocytes, inwhich chemoattractant-induced polarization worsened. Monocytes of healthy individuals up-regulated CD49e and d integrins and their capability to bindFITC-labelled FN after adherence to a FN-coated plate, as well as enhancing their capability to generate T cell-stimulatory VCs. Monocytes of TAID patients did not. These data indicate that integrin- (and chemokine-) mediated functions arehampered in monocytes of TAID patients. Because integrin action is pivotalto processes such as monocyte adherence to endothelial cells, uropod formation, migration into tissues and differentiation into APC and macrophages, these defects might underly immune dysbalances important in thyroid autoimmune development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:36:59