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Titolo:
MEK, ERK, and p90RSK are present on mitotic tubulin in Swiss 3T3 cells - Arole for the MAP kinase pathway in regulating mitotic exit
Autore:
Willard, FS; Crouch, MF;
Indirizzi:
Australian Natl Univ, John Curtin Sch Med Res, Div Neurosci, Mol Signalling Grp, Canberra, ACT 2601, Australia Australian Natl Univ Canberra ACT Australia 2601 rra, ACT 2601, Australia
Titolo Testata:
CELLULAR SIGNALLING
fascicolo: 9, volume: 13, anno: 2001,
pagine: 653 - 664
SICI:
0898-6568(200109)13:9<653:MEAPAP>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; SPINDLE ASSEMBLY CHECKPOINT; EPIDERMAL GROWTH-FACTOR; CLEAVING XENOPUS-EMBRYOS; S6 KINASE; MEIOTIC MATURATION; G(2)/M TRANSITION; PHASE-TRANSITION; METAPHASE ARREST; CYCLIN D1;
Keywords:
mitosis; MAP kinase; MEK; ERK; RSK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Willard, FS Australian Natl Univ, John Curtin Sch Med Res, Div Neurosci, Mol Signalling Grp, GPO Box 334, Canberra, ACT 2601, Australia Australian Natl Univ GPO Box 334 Canberra ACT Australia 2601 a
Citazione:
F.S. Willard e M.F. Crouch, "MEK, ERK, and p90RSK are present on mitotic tubulin in Swiss 3T3 cells - Arole for the MAP kinase pathway in regulating mitotic exit", CELL SIGNAL, 13(9), 2001, pp. 653-664

Abstract

The mitogen-activated protein (MAP) kinase pathway has been implicated in cell cycle control for some time. Several reports have suggested a role forthis pathway in growth factor stimulation of DNA synthesis, while other reports have proposed a role in the transition of cells through mitosis. Here, we have examined the potential involvement of the extracellular signal-related kinase (ERK)1/2 MAP kinases, their upstream regulators, and downstream effectors in the regulation of mitosis. Inhibition of MAP kinase/ERK kinase (MEK) activity reduced the serum-stimulated DNA synthesis and proliferation of Swiss 3T3 cells. To study the potential mechanisms of this effect, we examined the subcellular localization of members of the MAP kinase pathway including regulators (MEK1/2), substrates (90-kDa ribosomal S6 kinases (RSKs): RSK1, RSK2 and RSK3), and ERK itself. We show that there is enrichment of ERK, MEK, and the RSK enzymes on both the spindle and midbody tubulin of dividing cells. Inhibition of MEK1/2 activity in cells released from mitotic arrest results in an inability of cells to complete mitosis. This failure to exit mitosis correlated with altered cyclin-dependent kinase (cdk) activities. Thus, the MAP kinase pathway may act to coordinate passage through mitosis in Swiss 3T3 fibroblasts by regulation of cdk activity. (C) 2001Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:04:37