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Titolo:
Expression of constitutively active Rab5 uncouples maturation of the Salmonella-containing vacuole from intracellular replication
Autore:
Baldeon, ME; Ceresa, BP; Casanova, JE;
Indirizzi:
Univ Virginia, Hlth Sci Ctr, Dept Cell Biol, Charlottesville, VA 22908 USAUniv Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Scripps Clin & Res Inst, Dept Cell Biol, La Jolla, CA USA Scripps Clin & Res Inst La Jolla CA USA Dept Cell Biol, La Jolla, CA USA
Titolo Testata:
CELLULAR MICROBIOLOGY
fascicolo: 7, volume: 3, anno: 2001,
pagine: 473 - 486
SICI:
1462-5814(200107)3:7<473:EOCARU>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYSOSOMAL MEMBRANE-GLYCOPROTEINS; TYPHIMURIUM-CONTAINING VACUOLES; NUCLEOTIDE EXCHANGE FACTOR; HERMANSKY-PUDLAK-SYNDROME; ENDOCYTIC PATHWAY; EARLY ENDOSOME; MYCOBACTERIUM-TUBERCULOSIS; NUCLEAR RESPONSES; EPITHELIAL-CELLS; AP-3 ADAPTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Casanova, JE Univ Virginia, Hlth Sci Ctr, Dept Cell Biol, Box 800732, Charlottesville, VA 22908 USA Univ Virginia Box 800732 Charlottesville VA USA 22908 908 USA
Citazione:
M.E. Baldeon et al., "Expression of constitutively active Rab5 uncouples maturation of the Salmonella-containing vacuole from intracellular replication", CELL MICROB, 3(7), 2001, pp. 473-486

Abstract

The enteric bacterial pathogen Salmonella typhimurium enters and proliferates within both phagocytic and non-phagocytic host cells. Upon entry, the bacteria reside in membrane-bound vacuoles (SCVs) that mature with time, as evidenced by the sequential loss of early endosomal markers, followed by the selective recruitment of a number of lysosomal membrane glycoproteins (LAMPs). This remodelling process renders the SCVs non-fusogenic with lysosomes and is also thought to create a vacuolar environment permissive for replication. We demonstrate that disruption of the endocytic pathway by the expression of a constitutively active form of the small GTPase rab5 (rab5Q79L) significantly altered the biogenesis of the SCVs without inhibiting bacterial replication in HeLa cells. Expression of rab5Q79L caused the retention of early endosomal markers on SCVs and early acquisition of LAMP2, and led to an increase in the kinetics of intracellular replication. We also demonstrate that a significant fraction of LAMP2 in SCVs is derived from the cell surface via endocytosis rather than via the biosynthetic route. Further, infibroblasts lacking a functional AP3 adaptor complex, in which all newly synthesized LAMP is delivered to the cell surface, recruitment of LAMP to the SCVs remained unaffected. These findings raise the possibility that all the SCV-associated LAMP could be derived by endocytosis from the cell surface.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 20:10:02