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Titolo:
Leishmania donovani lipophosphoglycan causes periphagosomal actin accumulation: correlation with impaired translocation of PKC alpha and defective phagosome maturation
Autore:
Holm, A; Tejle, K; Magnusson, KE; Descoteaux, A; Rasmusson, B;
Indirizzi:
Linkoping Univ, Fac Hlth Sci, Dept Hlth & Environm, Div Med Microbiol, S-58185 Linkoping, Sweden Linkoping Univ Linkoping Sweden S-58185 obiol, S-58185 Linkoping, Sweden Univ Quebec, Inst Armand Frappier, INRS, Laval, PQ H7V 1B7, Canada Univ Quebec Laval PQ Canada H7V 1B7 pier, INRS, Laval, PQ H7V 1B7, Canada
Titolo Testata:
CELLULAR MICROBIOLOGY
fascicolo: 7, volume: 3, anno: 2001,
pagine: 439 - 447
SICI:
1462-5814(200107)3:7<439:LDLCPA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; PROTOZOAN PARASITE LEISHMANIA; SIGNAL-TRANSDUCTION; HUMAN NEUTROPHILS; HUMAN MONOCYTES; MARCKS FAMILY; FREE CALCIUM; MACROPHAGES; PHAGOCYTOSIS; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Holm, A Linkoping Univ, Fac Hlth Sci, Dept Hlth & Environm, Div Med Microbiol, S-58185 Linkoping, Sweden Linkoping Univ Linkoping Sweden S-58185 -58185 Linkoping, Sweden
Citazione:
A. Holm et al., "Leishmania donovani lipophosphoglycan causes periphagosomal actin accumulation: correlation with impaired translocation of PKC alpha and defective phagosome maturation", CELL MICROB, 3(7), 2001, pp. 439-447

Abstract

Lipophosphoglycan (LPG) is the major surface glycoconjugate of Leishmania donovani promastigotes. The repeating disaccharide-phosphate units of LPG are crucial for promastigote survival inside macrophages and establishment of infection. LPG has a number of effects on the host cell, including inhibition of PKC activity, inhibition of nitric oxide production and altered expression of cytokines. LPG also inhibits phagosomal maturation, a process requiring depolymerization of periphagosomal F-actin. In the present study, we have characterized the dynamics of F-actin during the phagocytosis of L. donovani promastigotes in J774 macrophages. We observed that F-actin accumulated progressively around phagosomes containing wild-type L. donovani promastigotes during the first hour of phagocytosis. Using LPG-defective mutants and yeast particles coated with purified LPG, we obtained evidence that this effect could be attributed to the repeating units of LPG. LPG also disturbed cortical actin turnover during phagocytosis. The LPG-dependent accumulation of periphagosomal F-actin correlated with an impaired recruitment ofthe lysosomal marker LAMP1 and PKC alpha to the phagosome. Accumulation ofperiphagosomal F-actin during phagocytosis of L. donovani promastigotes may contribute to the inhibition of phagosomal maturation by physically preventing vesicular trafficking to and from the phagosome.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:25:55