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Titolo:
Remodelling of the actin cytoskeleton is essential for replication of intravacuolar Salmonella
Autore:
Meresse, S; Unsworth, KE; Habermann, A; Griffiths, G; Fang, F; Martinez-Lorenzo, MJ; Waterman, SR; Gorvel, JP; Holden, DW;
Indirizzi:
Imperial Coll Sch Med, Ctr Mol Microbiol & Infect, Dept Infect Dis, LondonSW7 2AZ, England Imperial Coll Sch Med London England SW7 2AZ Dis, LondonSW7 2AZ, England Univ Med, CNRS, INSERM, Ctr Immunol Marseille Luminy, F-13288 Marseille 09, France Univ Med Marseille France 09 seille Luminy, F-13288 Marseille 09, France European Mol Biol Lab, D-69012 Heidelberg, Germany European Mol Biol Lab Heidelberg Germany D-69012 012 Heidelberg, Germany Univ Colorado, Hlth Sci Ctr, Denver, CO 80262 USA Univ Colorado Denver COUSA 80262 ado, Hlth Sci Ctr, Denver, CO 80262 USA
Titolo Testata:
CELLULAR MICROBIOLOGY
fascicolo: 8, volume: 3, anno: 2001,
pagine: 567 - 577
SICI:
1462-5814(200108)3:8<567:ROTACI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
III SECRETION SYSTEM; PHAGOCYTE NADPH-OXIDASE; PATHOGENICITY ISLAND 2; HOST-CELLS; INTRACELLULAR SURVIVAL; REGULATED EXOCYTOSIS; BACTERIAL VIRULENCE; CONTAINING VACUOLES; PROTEIN SECRETION; SEC13P COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Holden, DW Imperial Coll Sch Med, Ctr Mol Microbiol & Infect, Dept Infect Dis, Armstrong Rd, London SW7 2AZ, England Imperial Coll Sch Med Armstrong Rd London England SW7 2AZ land
Citazione:
S. Meresse et al., "Remodelling of the actin cytoskeleton is essential for replication of intravacuolar Salmonella", CELL MICROB, 3(8), 2001, pp. 567-577

Abstract

Maturation and maintenance of the intracellular vacuole in which Salmonella replicates is controlled by virulence proteins including the type III secretion system encoded by Salmonella pathogenicity island 2 (SPI-2). Here, we show that, several hours after bacterial uptake into different host cell types, Salmonella induces the formation of an F-actin meshwork around bacterial vacuoles. This structure is assembled de novo from the cellular G-actin pool in close proximity to the Salmonella vacuolar membrane. We demonstrate that the phenomenon does not require the Inv/Spa type III secretion system or cognate effector proteins, which induce actin polymerization during bacterial invasion, but does require a functional SPI-2 type III secretion system, which plays an important role in intracellular replication and systemic infection in mice. Treatment with actin-depolymerizing agents significantly inhibited intramacrophage replication of wild-type Salmonella typhimurium. Furthermore, after this treatment, wildtype bacteria were released into the host cell cytoplasm, whereas SPI-2 mutant bacteria remained within vacuoles. We conclude that actin assembly plays an important role in the establishment of an intracellular niche that sustains bacterial growth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:42:56