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Titolo:
Ultrastructural study of anterograde transport of glial cell line-derived neurotrophic factor from dorsal root ganglion neurons of rats towards the nerve terminal
Autore:
Ohta, K; Inokuchi, T; Gen, E; Chang, JW;
Indirizzi:
Kurume Univ, Sch Med, Dept Anat & Histol, Kurume, Fukuoka 8300011, Japan Kurume Univ Kurume Fukuoka Japan 8300011 , Kurume, Fukuoka 8300011, Japan
Titolo Testata:
CELLS TISSUES ORGANS
fascicolo: 4, volume: 169, anno: 2001,
pagine: 410 - 421
SICI:
1422-6405(2001)169:4<410:USOATO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSORY NEURONS; SCIATIC-NERVE; SUBSTANCE-P; SPINAL-CORD; PRIMARY AFFERENTS; FACTOR GDNF; IN-VIVO; RECEPTOR; RET; EXPRESSION;
Keywords:
neurotrophic factor; glial cell line-derived neurotrophic factor; vesicle, dense cored; axotomy; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Ohta, K Kurume Univ, Sch Med, Dept Anat & Histol, 67 Asahi Machi, Kurume, Fukuoka 8300011, Japan Kurume Univ 67 Asahi Machi Kurume Fukuoka Japan 8300011 011, Japan
Citazione:
K. Ohta et al., "Ultrastructural study of anterograde transport of glial cell line-derived neurotrophic factor from dorsal root ganglion neurons of rats towards the nerve terminal", CELLS T ORG, 169(4), 2001, pp. 410-421

Abstract

The glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic substance in the central and peripheral nervous systems. The present immunohistochemical study clarified the ultrastructural localization of GDNF-immunoreactive substance (GDNF-IR) accumulated at transfected sciatic nerve stumps and also at normal spinal dorsal horn, and has demonstrated that GDNF-IR products appear to be located in dense-cored vesicles within the axons. Furthermore, to determine the source of proximally accumulated GDNF inthe transected sciatic nerve, we attempted a transection and a double ligation maneuver involving the sciatic nerve. In the early period after the ligation (20 h), GDNF-IR fibers were observed in the proximal and distal segment of the ligations, but no immunoreactivities were detected in the middlesegment. On the other hand, at a late period (8 days) after the transection, GDNF-IR fibers had almost disappeared, but weak GDNF-IR was observed in Schwann cells in the proximal and distal stumps of transected nerve. These findings suggest that most of GDNF-IR was transported from the proximal or distal side in the early period, but was locally synthesized by Schwann cells around the ligations in the late period. Spinal rhizotomy caused prominent accumulation of GDNF-IR products at the cut end of the ganglion side of the dorsal root, but not at the ventral root. These results suggested that dorsal root ganglionic (DRG) sensory neurons are one of the origins of GDNF. The fact that small- to medium-sized DRG neurons show enhanced GDNR-IR after the colchicine treatment may support the above suggestion. In conclusion, the present results strongly suggest that a subgroup of DRG sensory neurons synthesized GDNF-containing dense-cored vesicles in the neuronal somataand anterogradely transports the vesicles to peripheral or central axon terminals. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:45:38