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Titolo:
Molecular weight, structure and shape of oat (1 -> 3),(l -> 4)-beta-D-glucan fractions obtained by enzymatic degradation with (1 -> 4)-beta-D-glucan 4-glucanohydrolase from Trichoderma reesei
Autore:
Roubroeks, JP; Andersson, R; Mastromauro, DI; Christensen, BE; Aman, P;
Indirizzi:
Swedish Univ Agr Sci, Dept Food Sci, SE-75007 Uppsala, Sweden Swedish UnivAgr Sci Uppsala Sweden SE-75007 i, SE-75007 Uppsala, Sweden Norwegian Univ Sci & Technol, Dept Biotechnol, NOBIPOL, N-7491 Trondheim, Norway Norwegian Univ Sci & Technol Trondheim Norway N-7491 1 Trondheim, Norway
Titolo Testata:
CARBOHYDRATE POLYMERS
fascicolo: 3, volume: 46, anno: 2001,
pagine: 275 - 285
SICI:
0144-8617(200111)46:3<275:MWSASO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-D-GLUCANS; ANION-EXCHANGE CHROMATOGRAPHY; AVENA-SATIVA L; BARLEY (1->3),(1->4)-BETA-D-GLUCAN; C-13-NMR SPECTROSCOPY; AQUEOUS-SOLUTIONS; LIGHT-SCATTERING; FINE-STRUCTURE; CELL-WALLS; OLIGOSACCHARIDES;
Keywords:
mixed-linkage (1 -> 3),(1 -> 4)-beta-D-glucan; oats; cellulase; molecular weight; shape; structure; kinetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Roubroeks, JP Swedish Univ Agr Sci, Dept Food Sci, POB 7051, SE-75007 Uppsala, Sweden Swedish Univ Agr Sci POB 7051 Uppsala Sweden SE-75007 weden
Citazione:
J.P. Roubroeks et al., "Molecular weight, structure and shape of oat (1 -> 3),(l -> 4)-beta-D-glucan fractions obtained by enzymatic degradation with (1 -> 4)-beta-D-glucan 4-glucanohydrolase from Trichoderma reesei", CARBOHY POL, 46(3), 2001, pp. 275-285

Abstract

Oat beta -glucan was partially degraded with (1-->4)-beta -D-glucan 4-glucanohydrolase for different periods of time. Weight average molecular weightand weight average intrinsic viscosity were obtained from SEC-RI-RALLS-Visc and ranged from 2200 to 213,900 g/mol and 7 to 316 ml/g, respectively. The viscosity equation determined [<(<eta>)over bar>](w) = 1.06 X 10(-2) (M) over bar (0.86)(w) indicated an extended random coil conformation. When thecoil was modelled as a worm-like chain a persistent length of 3.65 nm was obtained. The hydrolysis products identified after extensive degradation were glucose, cellobiose, laminaribiose, 4-O-beta -laminaribiosyl D-glucose, 4-O-beta -laminaribiosyl D-cellobiose and 3-O-beta -cellobiosyl D-cellobiose showing that other enzyme activities were present. The depolymerization kinetics suggested that longer sequences of consecutive (1-->4)-linkages represent fast hydrolysable sites and the presence of higher relative proportions of (1-->3)-linkages restricts the affinity of the enzyme. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:19:19