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Titolo:
A Glut4-vesicle marker protein, insulin-responsive aminopeptidase, is localized in a novel vesicular compartment in PC12 cells
Autore:
Thoidis, G; Kandror, KV;
Indirizzi:
Boston Univ, Sch Med, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 oston Univ, Sch Med, Boston, MA 02118 USA
Titolo Testata:
TRAFFIC
fascicolo: 8, volume: 2, anno: 2001,
pagine: 577 - 587
SICI:
1398-9219(200108)2:8<577:AGMPIA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-TRANSPORTER GLUT4; RAT ADIPOSE-CELLS; REGULATED MEMBRANE AMINOPEPTIDASE; 3T3-L1 ADIPOCYTES; SYNAPTIC VESICLE; PLASMA-MEMBRANE; GLUT4-CONTAINING VESICLES; SUBCELLULAR TRAFFICKING; STORAGE-VESICLES; DISTINCT;
Keywords:
Glut 4; insulin-responsive aminopeptidase; membrane traffic; PC12 cells; subcellular fractionation; vesicles;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Kandror, KV Boston Univ, Sch Med, Boston, MA 02118 USA Boston Univ BostonMA USA 02118 Sch Med, Boston, MA 02118 USA
Citazione:
G. Thoidis e K.V. Kandror, "A Glut4-vesicle marker protein, insulin-responsive aminopeptidase, is localized in a novel vesicular compartment in PC12 cells", TRAFFIC, 2(8), 2001, pp. 577-587

Abstract

Glut4-containing vesicles represent a regulated recycling compartment in insulin-sensitive fat and skeletal muscle cells, the nature and origin of which are not fully understood. In addition to Glut4 itself, these vesicles compartmentalize a number of proteins, at least one of which, insulin-responsive aminopeptidase, or IRAP, is completely colocalized with Glut4 in insulin-sensitive tissues. However, unlike Glut4, IRAP is expressed in a varietyof other tissues and cell lines. Here, we explored the intracellular localization of IRAP in the rat pheochromocytoma cell line PC12. We found that this protein is present in a distinct population of slowly recycling light vesicles. By gradient centrifugations, immunoadsorption and double immunofluorescent staining, these vesicles are different from transferrin-containingendosomes, small synaptic vesicles and secretory granules and may thus represent a novel compartment in PC12 cells. Glut4-GFP chimera transiently expressed in PC12 cells is targeted to IRAP-containing vesicles indicating that cotargeting of Glut4 and IRAP is not specific for adipocytes and myocytes, but is faithful in a foreign cell type. We suggest that PC12 cells may possess a novel type of a vesicular carrier that may represent the homolog ofGlut4-vesicles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 19:14:24