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Titolo:
Tacalcitol, an active vitamin D-3, induces nerve growth factor production in human epidermal keratinocytes
Autore:
Fukuoka, M; Sakurai, K; Ohta, T; Kiyoki, M; Katayama, I;
Indirizzi:
Teijin Inst Biomed Res, Hino, Tokyo 1918512, Japan Teijin Inst Biomed ResHino Tokyo Japan 1918512 ino, Tokyo 1918512, Japan Nagasaki Univ, Sch Med, Dept Dermatol, Nagasaki 852, Japan Nagasaki Univ Nagasaki Japan 852 Med, Dept Dermatol, Nagasaki 852, Japan
Titolo Testata:
SKIN PHARMACOLOGY AND APPLIED SKIN PHYSIOLOGY
fascicolo: 4, volume: 14, anno: 2001,
pagine: 226 - 233
SICI:
1422-2868(200107/08)14:4<226:TAAVDI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR MESSENGER-RNA; ADULT-RAT BRAIN; 1,25-DIHYDROXYVITAMIN D-3; DOUBLE-BLIND; TOPICAL APPLICATION; PSORIASIS; INDUCTION; RECEPTOR; CELLS; 1,24(OH)(2)D-3;
Keywords:
epidermal keratinocyte; K-TL-1; vitamin D-3; tacalcitol; nerve growth factor; peripheral neuropathy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Fukuoka, M Teijin Inst Biomed Res, 4-3-2 Asahigaoka, Hino, Tokyo 1918512, Japan Teijin Inst Biomed Res 4-3-2 Asahigaoka Hino Tokyo Japan 1918512
Citazione:
M. Fukuoka et al., "Tacalcitol, an active vitamin D-3, induces nerve growth factor production in human epidermal keratinocytes", SKIN PH APP, 14(4), 2001, pp. 226-233

Abstract

The human epidermal keratinocyte cell line K-TL-1, developed from a benignepidermal tumor, was cultured in the presence of the synthetic vitamin D-3analogue tacalcitol [1 alpha ,24(R)-dihydroxyvitamin D-3] to assess the effects on the production of nerve growth factor (NGF). Confluent K-TL-1 cells were cultured with 10(-8) M of tacalcitol. Supernatants and cell homogenates were collected and NGF concentrations were determined by enzyme-linked immunosorbent assay. The concentration of NGF in the supernatants of cultures treated with tacalcitol peaked within 24 h after the start of tacalcitoltreatment and remained stable for 96 h. This NGF induction caused by tacalcitol was dose-dependent, showing an ED50 between 10(-10) and 10(-9) M. Induction of NGF mRNA expression by tacalcitol was also observed by RT-PCR, indicating that tacalcitol induced NGF expression through transcriptional activation. These results suggest that active vitamin D-3 could treat peripheral neuropathy by inducing NGF production in the skin. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 06:48:16