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Titolo:
Simultaneous identification of GSTP1 Ile105 -> Val105 and Ala114 -> Val114substitutions using an amplification refractory mutation system polymerasechain reaction assay: studies in patients with asthma
Autore:
Hemmingsen, A; Fryer, AA; Hepple, M; Strange, RC; Spiteri, MA;
Indirizzi:
Keele Univ, N Staffordshire Hosp, Ctr Cell & Mol Med, Stoke On Trent ST4 6QG, Staffs, England Keele Univ Stoke On Trent Staffs England ST4 6QG ST4 6QG, Staffs, England
Titolo Testata:
RESPIRATORY RESEARCH
fascicolo: 4, volume: 2, anno: 2001,
pagine: 250 - 255
SICI:
1465-993X(2001)2:4<250:SIOGI->2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTATHIONE-S-TRANSFERASE; PI GENE; POLYMORPHISMS; SUSCEPTIBILITY; ASSOCIATION; LOCUS; VARIANTS; CANCER; LUNG; P1-1;
Keywords:
amplification refractory mutation system; asthma; bronchial hyperresponsiveness; GSTP1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Hemmingsen, A Keele Univ, N Staffordshire Hosp, Ctr Cell & Mol Med, Stoke On Trent ST4 6QG, Staffs, England Keele Univ Stoke On Trent Staffs EnglandST4 6QG s, England
Citazione:
A. Hemmingsen et al., "Simultaneous identification of GSTP1 Ile105 -> Val105 and Ala114 -> Val114substitutions using an amplification refractory mutation system polymerasechain reaction assay: studies in patients with asthma", RESPIR RES, 2(4), 2001, pp. 250-255

Abstract

Background: The glutathione S-transferase (GST) enzyme GSTP1 utilizes byproducts of oxidative stress. We previously showed that alleles of GSTP1 thatencode the Ile105 --> Val105 substitution are associated with the asthma phenotypes of atopy and bronchial hyperresponsiveness (BHR). However, a further polymorphic site (Ala114 --> Val114) has been identified that results in the following alleles: GSTP1*A (wild-type Ile105 --> Ala114), GSTP1*B (Val105 --> Ala114), GSTP1*C (Val105 --> Val114) and GSTP1*D (Ile105 --> Val114). Methods: Because full identification of GSTP1 alleles may identify stronger links with asthma phenotypes, we describe an amplification refractory mutation system (ARMS) assay that allows identification of all genotypes. We explored whether the GSTP1 substitutions influence susceptibility to asthma,atopy and BHR. Results: Among 191 atopic nonasthmatic, atopic asthmatic and nonatopic nonasthmatic individuals, none had the BD, CD, or DD genotypes. GSTP1 BC was significantly associated with reduced risk for atopy (P = 0.031). Compared with AA, trend test analysis identified a significant decrease in the frequency of GSTP1 BC with increasing severity of BHR (P = 0.031). Similarly, thefrequency of GSTP1 AA increased with increasing BHR. Conclusion: These data suggest that GSTP1*B and possibly GSTP1*C are protective against asthma and related phenotypes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 14:25:59