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Titolo:
Apoptotic activity of doxazosin on prostate stroma in vitro is mediated through an autocrine expression of TGF-beta 1
Autore:
Ilio, KY; Park, II; Pins, MR; Kozlowski, JM; Lee, C;
Indirizzi:
Northwestern Univ, Sch Med, Dept Urol, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 , Dept Urol, Chicago, IL 60611 USA Northwestern Univ, Sch Med, Dept Pathol, Chicago, IL USA Northwestern Univ Chicago IL USA , Sch Med, Dept Pathol, Chicago, IL USA
Titolo Testata:
PROSTATE
fascicolo: 3, volume: 48, anno: 2001,
pagine: 131 - 135
SICI:
0270-4137(20010801)48:3<131:AAODOP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-PROLIFERATION; RAT PROSTATE; GROWTH; TESTOSTERONE; HYPERPLASIA; INDUCTION; RECEPTOR; SYSTEM; ALPHA; DEATH;
Keywords:
stromal cells; Doxazosin; TGF-beta 1; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, C Northwestern Univ, Sch Med, Dept Urol, Tarry 11-715,303 E Chicago Ave, Chicago, IL 60611 USA Northwestern Univ Tarry 11-715,303 E Chicago Ave Chicago IL USA 60611
Citazione:
K.Y. Ilio et al., "Apoptotic activity of doxazosin on prostate stroma in vitro is mediated through an autocrine expression of TGF-beta 1", PROSTATE, 48(3), 2001, pp. 131-135

Abstract

BACKGROUND. Doxazosin, an alpha-adrenergic antagonist, has been shown to induce apoptosis in prostatic stromal cells. The mechanism of this apoptoticaction by Doxazosin remains undefined. The present study was carried out to demonstrate that the effect of Doxazosin on apoptosis of prostate stromalcells is mediated through an autocrine action of TGF-beta1. METHODS. Primary cultures of human prostate cells were treated with varying concentrations of Doxazosin (0, 0.1, 1, 10, and 100 muM) for a period up to 3 days. At the end of the 3-day culture, cell numbers were counted. Apoptosis was assessed by a colorimetric terminal deoxyribonucleotide transferase labeling technique. TGF-beta1 was determined by enzyme-linked immunosorbent assay (ELISA). RESULTS. Compared to control cultures, cell numbers were significantly decreased as much as 68.4% in cultures treated with 10 muM of Doxazosin after 3 days incubation, while apoptosis increased by 64.7% in cultures treated with the same concentration of Doxazosin after 24 h. This decrease in cell number was reversed when antibody to TGF-beta1 was added to these cultures. Addition of TGF-beta1 (0, 1.0, and 10 ng/mL) to the cultures also decreasedthe cell numbers. Quantitation of TGF-beta1 in lysates of cells by ELISA revealed that the cells treated with Doxazosin (10 VM) produced as much as 62.5% more TGF-beta1 than in that of untreated cells. CONCLUSIONS. These results demonstrate that the apoptotic effect of Doxazosin on human prostatic stromal cells is mediated through an autocrine production of TGF-beta1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 13:18:41