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Titolo:
Deaggregation is an integral component of the response of platelets to ADPin vitro: kinetic studies of literature and original data
Autore:
Maayani, S; Tagliente, TM; Schwarz, T; Craddock-Royal, B; Alcala, C; Marrero, G; Martinez, R;
Indirizzi:
Mt Sinai Med Ctr, Dept Anesthesiol, New York, NY 10029 USA Mt Sinai Med Ctr New York NY USA 10029 nesthesiol, New York, NY 10029 USA
Titolo Testata:
PLATELETS
fascicolo: 5, volume: 12, anno: 2001,
pagine: 279 - 291
SICI:
0953-7104(200108)12:5<279:DIAICO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BLOOD-PLATELETS; INDUCED REFRACTORY STATE; CYCLIC FLOW VARIATIONS; ISOLATED RABBIT AORTA; DISAGGREGATION KINETICS; AGGREGATED PLATELETS; ADRENERGIC AGONIST; WHOLE-BLOOD; CLOPIDOGREL; RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Maayani, S Mt Sinai Med Ctr, Dept Anesthesiol, Box 1010,1 Gustave L Levy Pl, New York, NY 10029 USA Mt Sinai Med Ctr Box 1010,1 Gustave L Levy Pl NewYork NY USA 10029
Citazione:
S. Maayani et al., "Deaggregation is an integral component of the response of platelets to ADPin vitro: kinetic studies of literature and original data", PLATELETS, 12(5), 2001, pp. 279-291

Abstract

Adenosine diphosphate (ADP) is recognized as an important mediator of platelet aggregation. Transient aggregation at low (less than or equal to1 M-mu), and sustained aggregation at higher ADP concentrations are consistently observed. Dissociation of platelet aggregates has been described and may explain the reversible component of the aggregation response. We hypothesizedthat the net aggregation response to ADP in vitro results from the concurrent activation of two opposing processes, aggregation and deaggregation. Different purinergic receptor subtypes may mediate these effects. To test this hypothesis and its generalizability, we performed a kinetic analysis of representative published ADP-induced aggregation responses supplemented withoriginal data from our laboratory. A four-compartment kinetic model was used to estimate k(3), a rate constant of deaggregation. Two model-independent parameters, the magnitude of the aggregation response ( DOD) and the timeto reach maximal aggregation (t(peak)) were also assessed. Greater sustained aggregation at higher ADP concentrations was consistently associated with increased DeltaOD and t(peak) but decreased k(3) values. These relationships were independent of type of platelet preparation or experimental conditions and not due to ADP receptor desensitization. Conversely, blockade of the P2Y(12) receptor subtype (ticlopidine, clopidogrel or 2-MeS-AMP) decreased DeltaOD and t(peak) but increased k(3) values. This supports the presence of active deaggregation which is decelerated by activation of the P2Y(12)receptor subtype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 22:07:56