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Titolo:
The utility of green fluorescent protein in transgenic plants
Autore:
Stewart, CN;
Indirizzi:
Univ N Carolina, Dept Biol, Greensboro, NC 27402 USA Univ N Carolina Greensboro NC USA 27402 pt Biol, Greensboro, NC 27402 USA
Titolo Testata:
PLANT CELL REPORTS
fascicolo: 5, volume: 20, anno: 2001,
pagine: 376 - 382
SICI:
0721-7714(200107)20:5<376:TUOGFP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
AGROBACTERIUM-MEDIATED TRANSFORMATION; GENE-EXPRESSION; SCREENABLE MARKER; VISUAL MARKER; VITAL MARKER; CODON USAGE; CELLS; GFP; MUTATIONS; REPORTER;
Keywords:
fluorescent proteins; GFP; gene expression; marker genes; plant transformation;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Stewart, CN Univ N Carolina, Dept Biol, Greensboro, NC 27402 USA Univ N Carolina Greensboro NC USA 27402 ensboro, NC 27402 USA
Citazione:
C.N. Stewart, "The utility of green fluorescent protein in transgenic plants", PL CELL REP, 20(5), 2001, pp. 376-382

Abstract

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as those including the S65T chromophore mutation and those with dual excitation peaks, are discussed. The review also focuses on the utility of GFP as a visual selectable marker in aiding the plant transformationprocess; GFP has been more important in monocot transformation compared with dicot transformation. Finally, the potential utility of new fluorescent proteins is speculated upon.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:43:28