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Titolo:
Sputum processing for evaluation of inflammatory mediators
Autore:
Kim, JS; Hackley, GH; Okamoto, K; Rubin, BK;
Indirizzi:
Wake Forest Univ, Sch Med, Dept Pediat, Winston Salem, NC 27157 USA Wake Forest Univ Winston Salem NC USA 27157 , Winston Salem, NC 27157 USA
Titolo Testata:
PEDIATRIC PULMONOLOGY
fascicolo: 2, volume: 32, anno: 2001,
pagine: 152 - 158
SICI:
8755-6863(200108)32:2<152:SPFEOI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT HUMAN DNASE; OBSTRUCTIVE PULMONARY-DISEASE; CYSTIC-FIBROSIS; BRONCHOALVEOLAR LAVAGE; AIRWAY INFLAMMATION; CHRONIC-BRONCHITIS; TNF-ALPHA; INTERLEUKIN-8; ASTHMA; FLUID;
Keywords:
DNA; interleukin-8; myeloperoxidase; neutrophils; inflammation; dornase alfa; dithiothreitol; cystic fibrosis; chronic bronchitis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Rubin, BK Wake Forest Univ, Sch Med, Dept Pediat, Med Ctr Blvd, Winston Salem, NC 27157 USA Wake Forest Univ Med Ctr Blvd Winston Salem NC USA 27157 157 USA
Citazione:
J.S. Kim et al., "Sputum processing for evaluation of inflammatory mediators", PEDIAT PULM, 32(2), 2001, pp. 152-158

Abstract

Neutrophil-dominated inflammation is prominent in the cystic fibrosis (CF)and chronic bronchitis (CB) airways. We assessed the degree of airway inflammation by measuring the sputum concentrations of interleukin (IL)-8, myeloperoxidase (MPO), and deoxyribonucleic acid (DNA). We determined the relationship among the concentrations of these mediators and investigated methodological problems that may be responsible for reported variability in measurements. Sputa obtained from 31 patients were solubilized with phosphate-buffered saline, dithiothreitol (DTT) (0.1 % or 1 %), or dornase alfa (0.2 mg/mL). The sputum concentration of IL-8 and MPO was measured by enzyme-linked immunosorbent assay (ELISA), and DNA was measured using microfluorimetry. There was a significant relationship among sputum IL-8, MPO, and DNA. For MPO (means +/- SD), CF was 1,392 +/- 771 vs. CB at 75 +/- 65 mcg/mL; P < 0.0001. For IL-8: CF was 239 +/- 154 vs. CB at 121 +/- 108 ng/mL; P= 0.0002. For DNA, CF was 1.707 +/- 1.25 vs. CB at 0.184 +/- 0.272 mg/mL; P < 0.0001. The MPO concentration in CF sputum was approximately double after in vitrotreatment with dornase alfa (P < 0.0001). There is a greater concentration of IL-8, MPC, and DNA in CF than in CB sputa. There is a significant relationship among these inflammatory markers in sputum. DNA polymers bind myeloperoxidase in the sputum, and we speculatethat treatment with dornase alfa may remove a source of MPO inhibition. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 12:28:01