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Titolo:
Permeability barrier properties of oral keratinocyte cultures: a model of intact human oral mucosa
Autore:
Selvaratnam, L; Cruchley, AT; Navsaria, H; Wertz, PW; Hagi-Pavli, EP; Leigh, IM; Squier, CA; Williams, DM;
Indirizzi:
Queen Marys Sch Med & Dent, Ctr Cutaneous Dis Res, London E1 2AT, England Queen Marys Sch Med & Dent London England E1 2AT London E1 2AT, England Univ Iowa, Dow Inst Dent Res, Iowa City, IA USA Univ Iowa Iowa City IA USA iv Iowa, Dow Inst Dent Res, Iowa City, IA USA
Titolo Testata:
ORAL DISEASES
fascicolo: 4, volume: 7, anno: 2001,
pagine: 252 - 258
SICI:
1354-523X(200107)7:4<252:PBPOOK>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN EPIDERMAL-KERATINOCYTES; HUMAN BUCCAL EPITHELIUM; STRATUM-CORNEUM LIPIDS; AIR-LIQUID INTERFACE; RECONSTRUCTED EPIDERMIS; TR146 CELLS; IN-VITRO; WATER PERMEABILITY; DRUG-DELIVERY; HUMAN SKIN;
Keywords:
permeability; oral mucosa; culture; lipid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Cruchley, AT Queen Marys Sch Med & Dent, Ctr Cutaneous Dis Res, London E1 2AT, England Queen Marys Sch Med & Dent London England E1 2AT T, England
Citazione:
L. Selvaratnam et al., "Permeability barrier properties of oral keratinocyte cultures: a model of intact human oral mucosa", ORAL DIS, 7(4), 2001, pp. 252-258

Abstract

OBJECTIVES: The aim of this study was to establish whether an in vitro model of human oral mucosa had similar permeability characteristics to normal oral mucosa. Such a model would have considerable value as an alternative to the use of mucosal biopsies in studies of transmucosal drug delivery. MATERIALS AND METHODS: Keratinocytes obtained from buccal mucosa, hard palate and abdominal skin were seeded onto inert collagen membranes (Cellagen Discs) or dead de-epidermised dermis (DDED) and grown either as submerged or air-liquid interface cultures. Subsequently the ultrastructural characteristics, permeability to water and barrier lipid content of the epithelial cultures were assessed and compared with samples of intact mucosa and skin. RESULTS: All the cultures stratified into multilayered epithelia and displayed features of differentiation including tonofilaments, desmosomes and membrane coating granules. The permeability characteristics and barrier lipidcontent of the oral mucosal cultures resembled those of intact mucosa. By contrast, epidermal keratinocytes failed to produce a permeability barrier comparable with that of skin and had low levels of barrier associated lipids. CONCLUSIONS: Cultures of human oral mucosal keratinocytes obtained from healthy adults develop similar permeability properties and barrier lipid composition to their site of origin. This model system may be useful for the evaluation of local and systemic oral mucosal drug delivery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 09:49:19