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Titolo:
Ischemia increases detectable endothelial nitric oxide synthase in rat andhuman myocardium
Autore:
Bloch, W; Mehlhorn, U; Krahwinkel, A; Reiner, M; Dittrich, M; Schmidt, A; Addicks, K;
Indirizzi:
Univ Cologne, Inst Anat 1, D-50931 Cologne, Germany Univ Cologne CologneGermany D-50931 st Anat 1, D-50931 Cologne, Germany Univ Cologne, Clin Thorac & Cardiovasc Surg, D-50931 Cologne, Germany UnivCologne Cologne Germany D-50931 vasc Surg, D-50931 Cologne, Germany
Titolo Testata:
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
fascicolo: 4, volume: 5, anno: 2001,
pagine: 317 - 333
SICI:
1089-8603(200108)5:4<317:IIDENO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-BLOCKADE; CARDIAC MYOCYTES; HEART; RELEASE; CELLS; HYPOXIA; PATHOPHYSIOLOGY; TRANSCRIPTION; REPERFUSION; ACTIVATION;
Keywords:
endothelial nitric oxide synthase; immunocytochemistry; ischemia; myocardium; cardiac surgery; NADPH-diaphorase; cardioplegia; heart perfusion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Bloch, W Univ Cologne, Inst Anat 1, Joseph Stelzmann Str 9, D-50931 Cologne, Germany Univ Cologne Joseph Stelzmann Str 9 Cologne Germany D-50931 many
Citazione:
W. Bloch et al., "Ischemia increases detectable endothelial nitric oxide synthase in rat andhuman myocardium", NITRIC OXID, 5(4), 2001, pp. 317-333

Abstract

The aims of the present study were to establish if myocardial ischemia/reperfusion is associated with altered eNOS activity and if myocardial eNOS detection depends on its activity. We determined detectable eNOS in (1) myocardium of isolated perfused rat hearts subjected to either global or regional ischemia and (2) in left ventricular biopsies from patients undergoing two different methods of myocardial protection (i.e., intermittent cold bloodcardioplegia and continuous coronary perfusion with warm, beta -blocker-enriched blood) during coronary artery surgery. NOS detection was performed by NADPH-d staining and three eNOS-antibodies against different eNOS epitopes. In addition, activity dependent alteration of detectable eNOS was proofed by bradykinin treatment for 2 to 10 min. Ischemic and receptor mediated eNOS activation increased NADPH-d reactivity and eNOS immunoreaction as measured by antibodies against either amino acids of a central bovine eNOS domain or the human eNOS N-terminal end. In contrast, the antibody against the human eNOS C-terminal end exhibited no alteration of eNOS immunoreaction. The transient eNOS activation was associate wit increased cGMP content. In human myocardium subjected to ischemia during cardiac surgery we found that early reperfusion increases eNOS activity. These data demonstrate a strong association between myocardial ischemia/reperfusion and increased eNOS activity as measured by immunocytochemical staining against specific eNOS epitopes. It appears that eNOS activation and subsequent NO release may act as aregulatory system to counter balance the potentially deleterious effects of myocardial ischemia/reperfusion. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:54:29