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Titolo:
Up-regulated TGF-beta mRNA expression in splenic T cells of high IgA-pronemice: A murine model of IgA nephropathy with glomerulosclerosis
Autore:
Oyama, A; Muso, E; Ono, T; Matsushima, H; Yashiro, M; Suyama, K; Kamata, T; Nogaki, F; Kobayashi, I; Miyawaki, S; Yoshida, H; Sasayama, S;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, Kyoto 6068397, Japan Kyoto Univ Kyoto Japan 6068397 ovasc Med, Sakyo Ku, Kyoto 6068397, Japan Nippon Shinyaku Co Ltd, Res Labs, Kyoto 601, Japan Nippon Shinyaku Co LtdKyoto Japan 601 o Ltd, Res Labs, Kyoto 601, Japan Kitano Hosp, Inst Med Res, Div Nephrol, Osaka, Japan Kitano Hosp Osaka Japan o Hosp, Inst Med Res, Div Nephrol, Osaka, Japan
Titolo Testata:
NEPHRON
fascicolo: 4, volume: 88, anno: 2001,
pagine: 368 - 375
SICI:
0028-2766(200108)88:4<368:UTMEIS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; INCREASED MESSENGER-RNA; HUMAN B-CELLS; DDY MICE; PROLIFERATIVE GLOMERULONEPHRITIS; ANIMAL-MODEL; DEPOSITION; STRAIN; SWITCH; DIFFERENTIATION;
Keywords:
HIGA mice; flow cytometry; immunoglobulin A; transforming growth factor-beta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Muso, E Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, 54 KawaraCho, Kyoto 6068397, Japan Kyoto Univ 54 Kawara Cho Kyoto Japan 6068397 Kyoto 6068397, Japan
Citazione:
A. Oyama et al., "Up-regulated TGF-beta mRNA expression in splenic T cells of high IgA-pronemice: A murine model of IgA nephropathy with glomerulosclerosis", NEPHRON, 88(4), 2001, pp. 368-375

Abstract

Background/Aims: Recently, we established a high serum IgA-prone inbred (HIGA) mouse strain as a murine model of spontaneous IgA nephropathy by selective mating of high serum IgA ddY mice, and found that they showed enhancedproduction of glomerular extracellular matrix components with increased expression of TGF-beta mRNA and protein in the kidneys. In this study, we examined the roles of lymphocytes in the development of high serum IgA in thisstrain. Methods: We performed flow cytometric analyses of T and B cells insplenic mononuclear cells (SMNCs) from these mice using BALB/c mice as normal controls. We also compared serum TGF-beta1 concentrations and TGF-beta mRNA expression levels in the B-cell-depleted (T-cell-rich) fraction of SMNCs in these mice. Results: HIGA mice showed significantly fewer CD3-positive cells compared with BALB/c mice when young, but not when aged. The CD4/CD8 ratio of HIGA mice was lower than that of BALB/c mice, but this difference was not significant. Although the number of B220-positive cells did not vary significantly, the ratio of surface IgA-positive B cells was significantly increased in both young and adult HIGA mice. The B-cell-depleted SMNCs from HIGA mice exhibited higher levels of expression of TGF-beta and TGF-beta1 mRNA than controls from a young age, which were maintained throughout life, but there were no differences in PDGF, MCP-1 or bFGF expression between these two strains. In contrast to local mRNA expression, serum TGF-beta1 concentration was decreased in HIGA mice compared with BALB/c controls. Conclusion: These findings suggest that the mating procedure performed to establish HIGA mice selected for a unique phenotype of local up-regulation of TGF-beta production in the kidneys, as well as T cells that may contribute to both the early and consistently high serum IgA expression and enhanced production of renal extracellular matrix components in HIGA mice. Additionally, TGF-beta1 may act locally, not systemically, in a paracrine or autocrinemanner. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:32:53