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Titolo:
Reproducible but dynamic positioning of DNA in chromosomes during mitosis
Autore:
Dietzel, S; Belmont, AS;
Indirizzi:
Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 ell & Struct Biol, Urbana, IL 61801 USA
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 8, volume: 3, anno: 2001,
pagine: 767 - 770
SICI:
1465-7392(200108)3:8<767:RBDPOD>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
METAPHASE CHROMOSOMES; SISTER CHROMATIDS; VISUALIZATION; SCAFFOLD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Belmont, AS Univ Munich, Inst Anthropol & Humangenet, Richard Wagner St 10-1, D-80333 Munich, Germany Univ Munich Richard Wagner St 10-1 Munich Germany D-80333 any
Citazione:
S. Dietzel e A.S. Belmont, "Reproducible but dynamic positioning of DNA in chromosomes during mitosis", NAT CELL BI, 3(8), 2001, pp. 767-770

Abstract

How DNA is folded into chromosomes is unknown. Mitotic chromosome banding shows reproducibility in longitudinal compaction at a resolution of severalmegabase pairs, but it is less clear whether DNA sequences are targeted laterally to specific locations. The in vitro chromosome assembly of prokaryotic DNA(1) suggests that there is a lack of sequence requirements for chromosome condensation, implying an absence of DNA targeting. Protein extraction experiments indicate, however, that specific DNA sequences may bind to a chromosome scaffold(2,3). Chromosome banding patterns, using dyes with differential sequence specificity, have been interpreted to result from the alignment of AT-rich sequences in a partially helically folded chromosome scaffold(4). But fluorescence in situ hybridization experiments, perhaps owing to technical limitations, have shown at best only slight deviation from a random, lateral sequence distributions. Here we show that there is highly reproducible targeting of specific chromosome segments to the metaphase chromatid axis, but that these segments localize to the periphery of prophase and telophase chromosomes. Unfolding intermediates during anaphase and telophase suggest that sequence repositioning occurs through the global uncoilingof an underlying chromatid structure.

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Documento generato il 20/01/20 alle ore 04:51:08