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Titolo:
SV2 modulates the size of the readily releasable pool of secretory vesicles
Autore:
Xu, T; Bajjalieh, SM;
Indirizzi:
Univ Washington, Dept Physiol & Biophys, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 iol & Biophys, Seattle, WA 98195 USA Univ Washington, Dept Pharmacol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ept Pharmacol, Seattle, WA 98195 USA
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 8, volume: 3, anno: 2001,
pagine: 691 - 698
SICI:
1465-7392(200108)3:8<691:SMTSOT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL CHROMAFFIN CELLS; SNARE COMPLEX; NEUROTRANSMITTER RELEASE; MEMBRANE-FUSION; SYNAPTIC EXOCYTOSIS; KINETIC COMPONENTS; SYNAPTOTAGMIN-I; PROTEIN; CA2+; SYNTAXIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Bajjalieh, SM Huazhong Univ Sci & Technol, Sch Life Sci, Wuhan 430074, Peoples R China Huazhong Univ Sci & Technol Wuhan Peoples R China 430074 a
Citazione:
T. Xu e S.M. Bajjalieh, "SV2 modulates the size of the readily releasable pool of secretory vesicles", NAT CELL BI, 3(8), 2001, pp. 691-698

Abstract

The exocytosis of neurotransmitters is regulated by calcium and is plastic- features that suggest specialized regulation of the basic membrane trafficking process. Here we show that Synaptic Vesicle Protein 2 (SV2), a protein specific to neurons and endocrine cells, is required to maintain a pool of vesicles available for calcium-stimulated exocytosis. Direct measures ofexocytosis in adrenal chromaffin cells showed that the calcium-induced exocytotic burst, which operationally defines the readily releasable pool of vesicles, was significantly reduced in mice lacking SV2A. Burst kinetics were normal in cells from MA knockout animals, however, indicating that SV2 functions before the final events of fusion. Analyses of SDS-resistant SNARE (soluble NSF (N-ethylmaleimide-sensitive fusion) attachment protein receptor) complexes in brain tissue showed that loss of SV2A was associated with fewer SDS-resistant complexes. Our observations indicate that SV2 may modulate the formation of protein complexes required for fusion and therefore theprogression of vesicles to a fusion-competent state.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:18:41